COMBINATORIAL SIGNALING BY SONIC HEDGEHOG AND WNT FAMILY MEMBERS INDUCES MYOGENIC BHLH GENE-EXPRESSION IN THE SOMITE
COMBINATORIAL SIGNALING BY SONIC HEDGEHOG AND WNT FAMILY MEMBERS INDUCES MYOGENIC BHLH GENE-EXPRESSION IN THE SOMITE
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DOI：
10.1101/gad.9.23.2911
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发表时间：
1995-12-15
影响因子：
10.5
通讯作者：
LASSAR, AB
中科院分区：
文献类型：
--
作者：
MUNSTERBERG, AE;KITAJEWSKI, J;LASSAR, AB
我们之前已经证明，来自神经管和底板/脊索复合体的信号组合协同诱导未特异体节中肌源性 bHLH 基因和肌源性分化标记物的表达。在这项研究中，我们证明了在底板/脊索中表达的音刺猬（Shh）和在神经管背侧区域表达的 Wnt 家族成员的子集（Wnt-1、Wnt-3 和 Wnt-4）模仿这些组织的肌肉诱导活动。组合起来，Shh 和 Wnt-1 或 Wnt-3 足以在体外诱导闪族组织的肌生成。因此，我们提出体内肌节的形成可能是由腹侧中线组织（底板和脊索）分泌的Shh和背侧神经管分泌的Wnt配体的组合活性引导的。
We have demonstrated previously that a combination of signals from the neural tube and the floor plate/notochord complex synergistically induce the expression of myogenic bHLH genes and myogenic differentiation markers in unspecified somites. In this study we demonstrate that Sonic hedgehog (Shh), which is expressed in the floor plate/notochord, and a subset of Wnt family members (Wnt-1, Wnt-3, and Wnt-4), which are expressed in dorsal regions of the neural tube, mimic the muscle inducing activity of these tissues. In combination, Shh and either Wnt-1 or Wnt-3 are sufficient to induce myogenesis in semitic tissue in vitro. Therefore, we propose that myotome formation in vivo may be directed by the combinatorial activity of Shh secreted by ventral midline tissues (floor plate and notochord) and Wnt ligands secreted by the dorsal neural tube.