On-line immunoanalysis for bioprocess control.
On-line immunoanalysis for bioprocess control.
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用于生物过程控制的在线免疫分析。
DOI：
10.1016/0168-1656(93)90083-y
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发表时间：
1993
影响因子：
4.1
通讯作者：
H. Hoffmann
中科院分区：
文献类型：
--
作者：
C. Middendorf;B. Schulze;R. Freitag;T. Scheper;M. Howaldt;H. Hoffmann
免疫分析技术如ELISA通常用于检测培养过程中产生的蛋白质。由于传统ELISA方法耗时长，难以实现自动化，因此迫切需要一种合适的在线监测方法。将免疫分析与FIA技术相结合，提出了非均相免疫FIA法和比浊免疫FIA法。用这些FIA方法研究了以下蛋白质：耐热普鲁兰酶、IgG、抗凝血酶III和重组组织型纤溶酶原激活剂。在普鲁兰酶和单克隆小鼠IgG的情况下，浊度免疫FIA用于在线分析的培养过程。结果显示这些免疫分析的有效性和应用。
Immunoanalytical techniques such as ELISA are often used for the detection of proteins produced in cultivation processes. Owing to the difficulty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterogeneous and the turbidimetric immuno-FIA methods. The following proteins were investigated with these FIA methods: thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator. In the cases of pullulanase and monoclonal mouse IgG, the turbidimetric immuno-FIA was used for on-line analysis of the cultivation process. Results are presented here to demonstrate the effectiveness and application of these immunoanalyses.