RADIATION SENSITIVITY OF MESSENGER-RNA
RADIATION SENSITIVITY OF MESSENGER-RNA
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DOI：
10.1007/bf00267850
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
HERRLICH, P
中科院分区：
文献类型：
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作者：
PONTA, H;PFENNIGYEH, ML;HERRLICH, P
mRNA 功能因紫外线照射而失活。对于模板功能的失活，单位长度的 mRNA（以碱基为单位）比单位长度的 DNA（以碱基对为单位）敏感 2-3 倍。这些数据来自 4 个不修复 DNA 的实验系统：利福平处理的细胞中大肠杆菌 mRNA 的翻译、感染大肠杆菌中 T7 mRNA 的翻译、体内 f2 噬菌体 RNA 的翻译以及无染色体小细胞中稳定 mRNA 的翻译。对紫外线相对敏感性的比较与转录单位的紫外线作图技术相关，该技术在原核和真核遗传研究中越来越受欢迎。
mRNA function is inactivated by irradiation with UV light. A unit length mRNA (in bases) is 2-3 times more sensitive than a unit length of DNA (in base pairs) with respect to the inactivation of template function. These data stem from 4 experimental systems of all of which do not repair DNA: the translation of Escherichia coli mRNA in rifampicin-treated cells, of T7 mRNA in infected E. coli, of f2 phage RNA in vivo, and of stable mRNA in chromosomeless minicells. The comparison of relative sensitivies to UV is relevant to the technique of UV mapping of transcription units which enjoys increasing popularity in pro- and eukaryotic genetic research.