Phosphate levels influence the utilisation of rice rhizodeposition carbon and the phosphate-solubilising microbial community in a paddy soil
Phosphate levels influence the utilisation of rice rhizodeposition carbon and the phosphate-solubilising microbial community in a paddy soil
复制标题
DOI：
10.1016/j.soilbio.2017.12.014
复制
发表时间：
2018-03-01
影响因子：
9.7
通讯作者：
Zhu, Yong-Guan
中科院分区：
文献类型：
--
作者：
Long, Xi-En;Yao, Huaiying;Zhu, Yong-Guan
为了满足不断增长的人口对稻米食品的需求，迫切需要更好地了解土壤生物系统，以提高化肥的有效利用，特别是磷基肥料。微生物在有机化合物的分解和无机磷的增溶中发挥着重要作用。目前，磷有效性是否影响水稻根源碳的掺入以及增磷细菌的活性仍不确定。本文采用稳定同位素探测磷脂脂肪酸和DNA以及高通量测序方法，研究了在0（对照）、50和200 mg P2O5 kg（-1）处理的水稻土中活性p溶解菌和微生物群落。与对照（23.4-62.2%）相比，低磷条件下，16:1 ω - 7c、18:1 ω - 7c和18:1 ω - 9c（革兰氏阴性菌）的δ C-13值升高至69.2-167.4%，高磷条件下下降至6.6-58.6%。同样，在低磷输入的磷脂脂肪酸（16:1 ω - 7c， 18:1 ω - 7c和18:1 ω - 9c）中，C-13的富集也较高（1.04-1.47 ng g（-1）干重土壤）。高通量测序结果表明，低磷而非高磷输入促进了溶磷芽孢杆菌、aliicyclobacillus和Clostridium的生长。在C-12和C-13处理下，这些生物在轻DNA组分中的相对丰度显著增加，表明它们很少利用水稻根源碳作为碳源。相比之下，在磷输入条件下，溶磷根瘤菌、红螺旋菌和粘球菌更倾向于根源碳。碱性磷酸酶phoD和吡洛喹啉醌pqqC基因的群落分析和系统发育分析表明，根瘤菌和放线菌在水稻土壤磷素溶解中起主导作用。
To fulfil the demand of a growing population for rice food, a better understanding of soil biological systems is urgently required to improve the efficient use of chemical fertilisers, especially phosphate (P)-based fertilisers. Microorganisms play an important role in the decomposition of organic compounds and solubilisation of inorganic P. Presently, whether P availability affects the incorporation of rice root-derived carbon and the activity of P-solubilising bacteria remains uncertain. Herein, stable isotope probing of phospholipid fatty acids and DNA and high-throughput sequencing methods were used to investigate active P-solubilising bacteria and the microbial community in a paddy soil treated at 0 (controls), 50, and 200 mg P2O5 kg(-1). Compared with controls (23.4-62.2%), the delta C-13 values of 16:1 omega 7c, 18:1 omega 7c, and 18:1 omega 9c (Gram-negative bacteria) increased to 69.2-167.4% with low P input, but decreased to 6.6-58.6% under high P input. Similarly, higher C-13 enrichment (1.04-1.47 ng g(-1) dry weight soil) was observed in phospholipid fatty acids (16:1 omega 7c, 18:1 omega 7c and 18:1 omega 9c) with low P input. High-throughput sequencing indicated that low rather than high P input promoted the growth of P-solubilising Bacillus, Alicyclobacillus, and Clostridium. The relative abundance of these organisms increased significantly in light DNA fractions following both C-12 and C-13 treatments, suggesting they seldom use rice root-derived carbon as carbon sources. By contrast, P-solubilising Rhizobiales, Rhodospirillales, and Myxococcales preferred root-derived carbon under P input conditions. Community and phylogenetic analysis of genes related to P solubilisation (alkaline phosphatase phoD and pyrroloquinoline quinone pqqC) indicated a dominant role for Rhizobiales and Actinomycetales in paddy soil P solubilisation.