Real-time assessment of cytotoxicity by impedance measurement on a 96-well plate
Real-time assessment of cytotoxicity by impedance measurement on a 96-well plate
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DOI：
10.1016/j.snb.2006.10.024
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发表时间：
2007-05-21
影响因子：
8.4
通讯作者：
Rossi, F.
中科院分区：
文献类型：
--
作者：
Ceriotti, L.;Ponti, J.;Rossi, F.
在这项工作中，细胞粘附和细胞反应的金属化合物分类为细胞毒性和致癌性的评估电阻抗测量在96孔板原型。该系统在每个孔的底部集成叉指电极，以真实的实时监测由于细胞粘附而导致的电极/培养基界面处的电阻抗变化，电阻抗变化被认为是细胞活力的指标。在BALB/3 T3 A-31-1-1细胞系上在线评估亚砷酸钠、氯化镉和顺铂的细胞毒性，这是一种用于评估化学品的细胞毒性和致癌潜力的公知模型。根据标准化至对照的阻抗数据计算抑制半浓度（IC 50），并与用于研究化学品在BALB/3 T3细胞系模型中的基础细胞毒性的常规方法进行比较，例如集落形成效率（CFE）和3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四唑（MTT）测定。结果表明，即使CFE仍然更敏感，但通过三种测试评估的测试化学品诱导的细胞毒性行为相似。然而，基于阻抗的在线测量96孔板方法，在我们看来，是更省力和更容易的测试。钴作为微米和纳米颗粒的细胞毒性也进行了评估，揭示了该系统在线监测和高通量新化学品的可能性。(C)2006 Elsevier B. V.保留所有权利。
In this work cell adhesion and cellular response to metal compounds classified as cytotoxic and carcinogenic are assessed by electrical impedance measurement in a 96-well plate prototype. This system integrates at the bottom of each well interdigitated electrodes to monitor in real time the electrical impedance changes at the electrode/culture medium interface due to cell adhesion, considered as a index of cell viability. The cytotocixity of sodium arsenite, cadmium chloride and cis-platinum is assessed on-line on BALB/3T3 A-31-1-1 cell line, a well known model to evaluate cytotoxicity and carcinogenic potential of chemicals. The inhibitory half concentration (IC50) is calculated from the impedance data normalized to the control and compared with conventional methods used to study basal cytotoxicity of chemicals in BALB/3T3 cell line model, such as colony forming efficiency (CFE) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. The results suggest similar behavior of cytotoxicity induced by tested chemicals assessed by the three tests compared even if CFE remains the more sensitive. However, the impedance-based on-line measuring 96-well plate method is, on our point of view, the less laborious and easier test. The cytotoxicity of cobalt as micron and nanoparticles is also evaluated revealing the possibility for the system to monitor on-line and with high throughput new chemicals. (C) 2006 Elsevier B.V. All rights reserved.