RETROGRADE AXONAL-TRANSPORT OF NERVE GROWTH-FACTOR
RETROGRADE AXONAL-TRANSPORT OF NERVE GROWTH-FACTOR
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DOI：
10.1016/0006-8993(74)90536-8
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发表时间：
1974-01-01
期刊：
影响因子：
2.9
通讯作者：
IVERSEN, LL
中科院分区：
文献类型：
--
作者：
HENDRY, IA;STOCKEL, K;IVERSEN, LL
神经生长因子（NGF）从小鼠虹膜肾上腺素能神经末梢向颈上神经节节后交感神经元细胞体逆行轴突转运。在前房注射碘化神经生长因子（125I-NGF）后，注射侧和未注射侧颈上神经节的放射性积累相对较快，与皮下注射后的情况相同。然而，眼内注射4小时后，注射侧颈上神经节的放射性优先积累变得明显，注射侧和非注射侧神经节之间的这种差异逐渐增加，在16小时达到最大值。节后肾上腺素能纤维的横断以及先前眼内注射秋水秋碱消除了注射侧颈上神经节中125i - ngf的优先积累。而6-羟多巴胺对肾上腺素能神经末梢的破坏不影响其优先积累。结果表明，NGF的逆行轴突转运速率约为2.5 mm/h，与正向快速轴突转运一样，依赖于秋水仙碱敏感机制。然而，正如其他关于辣根过氧化物酶的研究所显示的那样，NGF的摄取可能不仅发生在神经末梢，也发生在末梢前。放射自显像研究通过显示注射侧颈上神经节中少量神经元的放射性明确定位，有力地支持了逆行转运的存在，而在非注射侧，放射性仅在整个神经节中弥漫性分布。
A retrograde axonal transport of nerve growth factor (NGF) from the adrenergic nerve terminals in the mouse iris to the cell bodies of postganglionic sympathetic neurones in the superior cervical ganglion has been demonstrated.After injection of iodinated nerve growth factor (125I-NGF) into the anterior eye-chamber there was a relatively rapid accumulation of radioactivity in the superior cervical ganglia on both injected and non-injected sides, as was the case after subcutaneous injection. However, 4 h after intraocular injection a preferential accumulation of radioactivity became apparent in the superior cervical ganglion on the injected side, and this difference between the ganglia on injected and non-injected sides gradually increased to a maximum at 16 h. Transection of the postganglionic adrenergic fibres as well as the prior intraocular injection of colchicine abolished the preferential accumulation of125I-NGF in the superior cervical ganglion of the injected side, whereas the destruction of adrenergic nerve terminals by 6-hydroxydopamine did not impair the preferential accumulation. It is concluded that the retrograde axonal transport of NGF, which was estimated to take place at a rate of about 2.5 mm/h, depends on a colchicine-sensitive mechanism as does the orthograde rapid axonal transport. However, the uptake of NGF may not only take place from the nerve terminals but also from the preterminal parts, as has been shown in other studies with horseradish peroxidase. Autoradiographic studies strongly supported the existence of a retrograde transport by showing a clear localization of radioactivity in a small number of neurones in the superior cervical ganglion on the injected side, whereas on the non-injected side there was only a diffuse distribution of radioactivity throughout the ganglion.