Altered food-anticipatory activity rhythm in Cryptochrome-deficient mice
Altered food-anticipatory activity rhythm in Cryptochrome-deficient mice
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DOI：
10.1016/j.neures.2005.03.003
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发表时间：
2005-06-01
影响因子：
2.9
通讯作者：
Shibata, S
中科院分区：
文献类型：
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作者：
Iijima, M;Yamaguchi, S;Shibata, S
在夜间活动的啮齿类动物中，限制白天进食 (RF) 会导致与进食相关的昼间运动活动，当停止进食时，这种活动会在接下来的 1-2 天内持续存在。伴随着这种预期行为，时钟基因（例如 mPer1 和 mPer2）的表达模式在肝脏和大脑皮层中从夜间模式变为昼间模式，但在视交叉上核 (SCN) 中却没有。以 mCry1 和 mCry2 基因为重要组成部分的分子发条装置是否参与食物预期昼夜节律尚不清楚。在这项研究中，我们研究了 mCRY 产物缺失对 RF 诱导的运动模式的影响。在野生型和 mCry1-1-mCry2-1-小鼠中，RF 导致白天活动增加，甚至在禁食后持续 2 天。然而，射频诱导的活性不太稳定，并且在突变小鼠中逐渐出现。饲养在持续黑暗下的小鼠或 SCN 损伤的野生型和突变型小鼠也获得了类似的结果。我们的数据表明，mCry 蛋白对于食物引起的振荡基本上是可有可无的。然而，值得注意的是，mCry 缺陷会影响 RF 诱导的预期运动活动的稳定性和发展。 (c) 2005 年爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
In nocturnal rodents, restricted feeding to daytime (RF) causes feeding-associated diurnal locomotor activity that persists for the next 1-2 days when food is withheld. Along with this anticipatory behavior, the expression pattern of clock genes such as mPer1 and mPer2 changes from a nocturnal to diurnal pattern in the liver and cerebral cortex but not in the suprachiasmatic nucleus (SCN). Whether the molecular clockwork, in which mCry1 and mCry2 genes are essential components, is involved in food-anticipatory circadian rhythms is unknown.In this study, we investigated the impact of the absence of mCRY products upon the locomotion pattern induced by RF. RF caused an increase in daytime activity that lasted even for 2 days after food was withheld, in wild-type and mCry1-1-mCry2-1-mice. However, RF-induced activity was less stable and appeared more gradually in mutant mice. Similar results were obtained with mice housed under constant darkness or with SCN-lesioned wild-type and mutant mice. Our data reveal that mCry proteins are basically dispensable for food-entrainable oscillation. However, it is also important to note that mCry deficiency affects the stability and development of RF-induced anticipatory locomotor activity. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.