Ionizing radiation induces cutaneous lipid remolding and skin adipocytes confer protection against radiation-induced skin injury
Ionizing radiation induces cutaneous lipid remolding and skin adipocytes confer protection against radiation-induced skin injury
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电离辐射诱导皮肤脂质重塑，皮肤脂肪细胞可防止辐射引起的皮肤损伤
DOI：
10.1016/j.jdermsci.2020.01.009
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发表时间：
2020-02-01
影响因子：
4.6
通讯作者：
Zhang, Shuyu
中科院分区：
文献类型：
--
作者：
Xiao, Yuji;Mo, Wei;Zhang, Shuyu
背景：放射性皮肤损伤是放射治疗和放射事故中的一个严重问题。皮肤脂肪代表了人体皮肤的主要结构成分。然而，皮肤脂肪和放射性皮肤损伤的进展之间的相互作用仍然在很大程度上unexplored.Objective：本研究旨在阐明皮肤脂肪和放射性皮肤损伤的进展之间的相互作用。通过RNA-Seq测定mRNA谱。通过磁共振成像（MRI）监测皮肤脂质质量，并通过液相色谱-质谱法（LC-MS）测量脂质分布。将从真皮和皮下白色脂肪组织（WAT）分离的人成熟脂肪细胞与人角质形成细胞（HaCaT）和皮肤成纤维细胞（WS 1）在transwell培养系统中共培养。结果：辐射通过下调多个途径调节皮肤脂质代谢。此外，辐射减少了皮肤脂肪量，改变了脂质代谢产物。与对照组相比，高脂饮食喂养的大鼠显示出对放射性皮肤损伤的抵抗力，表明皮肤脂质起着辐射防护作用。成熟脂肪细胞促进皮肤角质形成细胞和成纤维细胞的迁移，但不促进其增殖。软脂酸是皮肤组织中含量最丰富的脂肪酸，可促进WS 1细胞的迁移。此外，脂肪酸结合蛋白4（FABP 4）可以被纳入皮肤细胞，促进辐射皮肤成纤维细胞DNA损伤修复。结论：辐射诱导皮肤脂质重塑，皮肤脂肪细胞赋予对辐射诱导的皮肤损伤的保护作用。(C)2020日本皮肤病研究学会。Elsevier B. V.出版，保留所有权利。
Background: Radiation-induced skin injury is a serious concern during radiotherapyand radiation accidents. Skin fat represents the dominant architectural component of the human skin. However, the interplay between skin fat and the progression of radiation-induced skin injury remains largely unexplored.Objective: This study aims to elucidate the interplay between skin fat and the progression of radiation-induced skin injury.Methods: SD rats were irradiated with an electron beam. mRNA profiles were determined by RNA-Seq. The skin lipid mass was monitored by magnetic resonance imaging (MRI) and lipid profiles were measured by liquid chromatography-mass spectrometry (LC-MS). Human mature adipocytes isolated from dermal and subcutaneous white adipose tissues (WATs) were co-cultured with human keratinocytes (HaCaT) and skin fibroblasts (WS1) in the transwell culture system. Cell migration ability was measured by migration assay.Results: Radiation modulated cutaneous lipid metabolism by downregulating multiple pathways. Moreover, radiation decreased skin fat mass with altered lipid metabolite profiles. The rats fed with a high-fat diet showed resistance to radiogenic skin injury compared with that with a control diet, indicating that skin lipid plays a radioprotective role. Mature adipocytes promoted the migration but not the proliferation of co-cultured skin keratinocytes and fibroblasts. Palmitic acid, the most abundant fatty acid in skin tissues, facilitated the migration ofWS1 cells. Moreover, fatty acid-binding protein 4 (FABP4)could be incorporated into skin cells and promote DNA damage repair in irradiated skin fibroblasts.Conclusion: Radiation induces cutaneous lipid remolding, and skin adipocytes confer a protective role against radiation-induced skin injury. (C) 2020 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.