Characterization of alternative oxidase (AOX) gene expression in response to methyl salicylate and methyl jasmonate pre-treatment and low temperature in tomatoes
Characterization of alternative oxidase (AOX) gene expression in response to methyl salicylate and methyl jasmonate pre-treatment and low temperature in tomatoes
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DOI：
10.1016/j.jplph.2005.11.003
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发表时间：
2006-10-01
影响因子：
4.3
通讯作者：
Tian, Meisheng
中科院分区：
文献类型：
--
作者：
Fung, Raymond W. M.;Wang, Chien Y.;Tian, Meisheng
水杨酸甲酯（梅萨）蒸汽增加对Chitting伤害（Cl）在新鲜收获的粉红色番茄的抗性。交替氧化酶（AOX）的表达模式之前和期间的冷却表明，番茄果实的梅萨蒸汽预处理增加了AOX的转录水平。我们使用来自公共数据库的AOX的4个EST番茄克隆，它们属于植物中定义的两个明显相关的家族1和2。将三个克隆命名为LeAOX 1a、1b和1c，第四个克隆命名为LeAOX 2。RT-PCR检测结果表明，1a和1b基因在叶片、根和果实中均有表达，而1c基因在根中表达最强。来自AOX亚家族1的LeAOX 1a的RNA转录物比1b或1c的丰度大得多。通过RNA印迹分析，在冷藏番茄果实中检测到较长的AOX转录本，证实其为LeAOX 1a和LeAOX 1b基因的未剪接前体RNA转录本。在根中检测到LeAOX 1c基因的内含子剪接也受到冷藏的影响。AOX前体mRNA分子的这种选择性剪接事件发生在低温下，与mRNA丰度无关。参与RNA加工的几个关键基因（剪接因子：9 G8-SR和SF 2-SR，原纤蛋白和DEAD盒RNA解旋酶）的转录水平也受到储存温度变化的影响。本文还讨论了番茄果实冷害过程中AOX前体mRNA的异常剪接事件及其与RNA加工相关基因表达变化的关系。(c)2005年Elsevier GmbH。All rights reserved.
Methyl saticytate (MeSA) vapor increased resistance against chitting injury (Cl) in freshly harvested pink tomatoes. The expression patterns of alternative oxidase (AOX) before and during the chilling period demonstrated that pre-treatment of tomato fruit with MeSA vapor increased the transcript levels of AOX. We used 4 EST tomato clones of AOX from the public database that belong to two distinctly related families, 1 and 2 defined in plants. Three clones were designated as LeAOX1a, 1b and 1c and the fourth clone as LeAOX2. Using RT-PCR, 1a and 1b genes were found to be expressed in leaf, root and fruit tissues, but 1 c was expressed preferentially in roots. RNA transcript from LeAOX1a of AOX subfamily 1 was present in much greater abundance than 1b or 1c. The presence of longer AOX transcripts detected by RNA get blot analysis in cold-stored tomato fruit was confirmed to be the un-spliced prem-RNA transcripts of LeAOX1a and LeAOX1b genes. Intron splicing of LeAOX1c gene was also affected by cold storage when it was detected in roots. This alternative splicing event in AOX pre-mRNAs molecules occurred, preferentially at tow temperature, regardless of mRNA abundance. Transcript levels of several key genes involved in RNA processing (splicing factors: 9G8-SR and SF2-SR, fibrillarin and DEAD box RNA helicase) were also affected by changes in storage temperature. The aberrant splicing event in AOX pre-mRNA and its possible association with the change in expression of genes involved in RNA processing in tomato fruit having chilling disorder was discussed. (c) 2005 Elsevier GmbH. All rights reserved.