Functional analysis of GAT-specific T cell clones: H-2-restricted monoclonal T helper cells do not regulate expression of antibody isotypes.
Functional analysis of GAT-specific T cell clones: H-2-restricted monoclonal T helper cells do not regulate expression of antibody isotypes.
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GAT 特异性 T 细胞克隆的功能分析：H-2 限制性单克隆 T 辅助细胞不调节抗体同种型的表达。
DOI：
10.4049/jimmunol.129.1.217
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发表时间：
1982
影响因子：
4.4
通讯作者：
J. Thèze
中科院分区：
文献类型：
--
作者：
M. Seman;V. Zilberfarb;M. Gougeon;J. Thèze
我们从GAT致敏的BALB/c小鼠淋巴结细胞中获得了对poly（Glu 60，Ala 30，Tyr 10）（GAT）特异的T细胞克隆。克隆由载体特异性和H-2限制性的辅助细胞组成，需要半抗原-载体桥来激活B细胞。它们可以被认为是TH 1细胞。它们在体外过继转移中均能诱导不同同种型的抗体应答。克隆获得的反应的同种型模式与多克隆T细胞获得的反应相同，并且不依赖于添加到培养物中的T辅助细胞的数量。因此，我们的观察表明，经典的TH 1细胞不调节亚型表达的二次反应。分析了独特型样决定簇在GAT特异性克隆上的表达。在BALB/c小鼠中制备的针对先前表征的BALB/c抗GAT抗体的同基因抗独特型血清（B658）显示特异性阻断来自BALB/c小鼠的GAT特异性T细胞系的辅助活性。在相同的实验条件下，BALB/c克隆的活性不受血清B658的影响。
We obtained T cell clones specific for poly (Glu60, Ala30, Tyr10) (GAT) from GAT-primed lymph node cells of BALB/c mice. The clones consisted of helper cells that were carrier-specific and H-2-restricted and required a hapten-carrier bridge to activate B cells. They may be considered TH1 cells. They could all induce antibody responses of various isotypes in adoptive transfer in vitro. Isotypic patterns of the responses obtained with the clones were identical to those obtained with polyclonal T cells and were not dependent on the number of T helper cells added to the culture. Therefore, our observations demonstrate that classic TH1 cells do not regulate isotype expression on secondary responses. The expression of idiotype-like determinants on the GAT-specific clones was assayed. A syngeneic anti-idiotypic serum (B658), prepared in BALB/c mice against BALB/c anti-GAT antibodies previously characterized, was shown to block specifically the helper activity of GAT-specific T cell lines from BALB/c mice. Under the same experimental conditions the activity of none of the BALB/c clones was affected by serum B658.