Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements
Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements
复制标题
DOI：
10.1105/tpc.9.11.1963
复制
发表时间：
1997-11-01
期刊：
影响因子：
11.6
通讯作者：
Guilfoyle, TJ
中科院分区：
文献类型：
--
作者：
Ulmasov, T;Murfett, J;Guilfoyle, TJ
A highly active synthetic auxin response element (AuxRE), referred to as DR5, was created by performing site-directed mutations in a natural composite AuxRE found in the soybean GH3 promoter. DR5 consisted of tandem direct repeats of 11 bp that included the auxin-responsive TGTCTC element. The DR5 AuxRE showed greater auxin responsiveness than a natural composite AuxRE and the GH3 promoter when assayed by transient expression in carrot protoplasts or in stably transformed Arabidopsis seedlings, and it provides a useful reporter gene for studying auxin-responsive transcription in wild-type plants and mutants. An auxin response transcription factor, ARF1, bound with specificity to the DR5 AuxRE in vitro and interacted with Aux/IAA proteins in a yeast two-hybrid system. Cotransfection experiments with natural and synthetic AuxRE reporter genes and effector genes encoding Aux/IAA proteins showed that overexpression of Aux/IAA proteins in carrot protoplasts resulted in specific repression of TGTCTC AuxRE reporter gene expression.
