INTRACELLULAR ROUTING OF TRANSFERRIN AND TRANSFERRIN RECEPTORS IN EPIDERMOID CARCINOMA A431-CELLS
INTRACELLULAR ROUTING OF TRANSFERRIN AND TRANSFERRIN RECEPTORS IN EPIDERMOID CARCINOMA A431-CELLS
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DOI：
10.1016/0092-8674(83)90235-0
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
HOPKINS, CR
中科院分区：
文献类型：
--
作者：
HOPKINS, CR
转铁蛋白过氧化物酶（Tfn-HRP）和转铁蛋白受体特异性抗体复合胶体金（ATR）被用来确定有关处理内化转铁蛋白受体复合物的细胞内室。主要的膜结合系统被确定在外周的细胞质和在aprotanuclear地区，从这些复合物的组件返回到细胞表面。时程研究表明，外周系统与短路有关，将配体和受体复合物再循环回细胞的上表面。胞核隔室是一个较长回路的一部分，该回路将一些受体传递到基底表面，而将其他受体与配体一起沿着到溶酶体。
Transferrin peroxidase (Tfn-HRP) and a transferrin receptor-specific antibody complexed to colloidal gold (ATR) were used to identify the intracellular compartments concerned with processing internalized transferrin-receptor complexes. Major membrane-bound systems were identified in the peripheral cytoplasm and in the juxtanuclear area, from which components of these complexes are returned to the cell surface. Time course studies indicate that the peripheral system is concerned with a short circuit, recycling ligand and receptor complexes back to the upper surface of the cell. The juxtanuclear compartment is part of a longer circuit that routes some receptors to the basal surface and others, along with ligand, to the lysosome.