A breakdown of Brassica self-incompatibility in ARC1 antisense transgenic plants
A breakdown of Brassica self-incompatibility in ARC1 antisense transgenic plants
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DOI：
10.1126/science.286.5445.1729
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发表时间：
1999-11-26
期刊：
影响因子：
56.9
通讯作者：
Goring, DR
中科院分区：
文献类型：
--
作者：
Stone, SL;Arnoldo, M;Goring, DR
自交不亲和性，即拒绝自体花粉，是开花植物防止近亲繁殖最普遍的机制。在油菜中，S受体激酶(SRK)参与了自交不亲和反应，但涉及SRK的分子机制尚不清楚。一种可能的SRK下游效应因子是ARC1，它是一种与SRK激酶域结合的蛋白质。这表明自交不亲和甘蓝型油菜W1品系中Arc1信使RNA水平的抑制与自交不亲和性的部分破坏有关，从而导致种子产生。这为Arc1是油菜自交不亲和反应的积极效应提供了强有力的证据。
Self-incompatibility, the rejection of self pollen, is the most widespread mechanism by which flowering plants prevent inbreeding. In Brassica, the S receptor kinase (SRK) has been implicated in the self-incompatibility response, but the molecular mechanisms involving SRK are unknown. One putative downstream effector for SRK is ARC1, a protein that binds to the SRK kinase domain. Here it is shown that suppression of ARC1 messenger RNA levels in the self-incompatible Brassica napus W1 line is correlated with a partial breakdown of self-incompatibility, resulting in seed production. This provides strong evidence that ARC1 is a positive effector of the Brassica self-incompatibility response.