Gene expression signatures that classify the mode of invasion of primary oral squamous cell carcinomas
Gene expression signatures that classify the mode of invasion of primary oral squamous cell carcinomas
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DOI：
10.1002/mc.20431
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发表时间：
2008-10-01
影响因子：
4.6
通讯作者：
Yamamoto, Mikio
中科院分区：
文献类型：
--
作者：
Kondoh, Nobuo;Ishikawa, Toshio;Yamamoto, Mikio
目的研究进展性口腔鳞状细胞癌（OSCC）的分子特征，建立新的诊断模型。从淋巴结阳性和阴性患者的原发性OSCC中分离总RNA，并用于cDNA微阵列分析。为了确定代表恶性表型的标记基因，通过定量逆转录-PCR（QRT-PCR）进一步检测其在64例OSCC组织中的表达。使用Fisher线性判别分析（LDA）拟合逐步增量法，我们建立了歧视性预测模型。这些模型的稳定性进行了检查，使用留一交叉验证。进行免疫组织化学分析。在芯片分析的16600个可能的靶cDNA中，83个基因表现出显著差异信号（>2倍）。我们进一步鉴定了53个与Yamamoto-Kohama（YKs）侵袭OSCC模式有关的标记基因（P
To identify molecular signatures and establish a new diagnostic model for progressive oral squamous cell carcinoma (OSCC). Total RNAs were isolated from primary OSCCs from both node-positive and -negative patients and used in cDNA microarray analysis. To identify marker genes representing a malignant phenotype, their expression was further examined by quantitative reverse transcription-PCR (QRT-PCR) in 64 OSCC tissues. Using Fisher's linear discriminant analysis (LDA) fitted with a stepwise increment method, we created discriminatory predictor models. The stability of these models was examined using leave-one-out cross validation. immunohistochemical analysis was performed. Among the 16 600 possible target cDNAs in the array analysis, 83 genes demonstrated significantly differential signals (>2-fold). We further identified 53 marker genes that can be implicated in the Yamamoto-Kohama's (YKs) mode of invasion for OSCCs (P