Evidence for cell-surface association between fusin and the CD4-gp120 complex in human cell lines
Evidence for cell-surface association between fusin and the CD4-gp120 complex in human cell lines
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DOI：
10.1126/science.274.5287.602
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发表时间：
1996-10-25
期刊：
影响因子：
56.9
通讯作者：
Golding, H
中科院分区：
文献类型：
--
作者：
Lapham, CK;Ouyang, J;Golding, H
参与人类免疫缺陷病毒 1 型进入细胞的辅助细胞表面分子最近已被鉴定，并被证明属于趋化因子受体家族。在 37°C 下用可溶性单体 gp120 处理人类细胞系，诱导表面 CD4-gp120 复合物与 45 千道尔顿蛋白之间的关联，该蛋白可被佛波醇酯佛波醇下调 12-肉豆蔻酸酯13-乙酸酯。这三种蛋白质与 CD4 或 gp120 的抗体从细胞膜上共沉淀，45 千道尔顿蛋白质与十二烷基硫酸钠凝胶上的融合蛋白共迁移，并在蛋白质免疫印迹中与融合蛋白的兔抗血清反应，没有 45 千道尔顿蛋白质可以从类似处理的非人类细胞中共沉淀，然而， 用牛痘-融合蛋白重组病毒 (vCBYF1) 感染 3T3.CD4.401 细胞，然后进行 gp120 处理，导致融合蛋白和 CD4.401 分子从细胞膜上共沉淀。这些数据共同为融合蛋白和细胞膜上的 CD4-gp120 复合物之间的物理关联提供了证据。
Accessory cell-surface molecules involved in the entry of human immunodeficiency virus-type 1 into cells have recently been identified and shown to belong to the family of chemokine receptors, Treatment of human cell lines with soluble monomeric gp120 at 37 degrees C induced an association between the surface CD4-gp120 complex and a 45-kilodalton protein, which can be down-modulated by the phorbol ester phorbol 12-myristate 13-acetate. The three proteins were coprecipitated from the cell membranes with antibodies to CD4 or to gp120, The 45-kilodalton protein comigrated with fusin on sodium dodecyl sulfate gels and reacted with rabbit antisera to fusin in protein immunoblots, No 45-kilodalton protein could be coprecipitated from similarly treated nonhuman cells, However, infection of 3T3.CD4.401 cells with vaccinia-fusin recombinant virus (vCBYF1), followed by gp120 treatment, resulted in coprecipitation of fusin and CD4.401 molecules from their membranes. Together these data provide evidence for physical association between fusin and the CD4-gp120 complex on cell membranes.