Modeling hybridoma cell metabolism using a generic genome-scale metabolic model of Mus musculus
Modeling hybridoma cell metabolism using a generic genome-scale metabolic model of Mus musculus
复制标题
DOI：
10.1021/bp0498138
复制
发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Nielsen, LK
中科院分区：
文献类型：
--
作者：
Sheikh, K;Förster, J;Nielsen, LK
基于注释的基因组数据、途径数据库和当前可用的生化和生理信息，提出了小家鼠的重构细胞代谢网络。虽然不完整，但它代表了第一次尝试收集和表征哺乳动物细胞的代谢网络的基因组数据的基础上。反应网络本质上是通用的，并试图捕获细胞的碳，能量和氮代谢。代谢反应在细胞质和线粒体之间进行区室化，包括区室和细胞外介质之间的运输反应。反应列表包括872种内部代谢物，涉及总共1220种反应，其中473种涉及已知的开放阅读框架。提出了重建模型的初始计算机分析。
The reconstructed cellular metabolic network of Mus musculus, based on annotated genomic data, pathway databases, and currently available biochemical and physiological information, is presented. Although incomplete, it represents the first attempt to collect and characterize the metabolic network of a mammalian cell on the basis of genomic data. The reaction network is generic in nature and attempts to capture the carbon, energy, and nitrogen metabolism of the cell. The metabolic reactions were compartmentalized between the cytosol and the mitochondria, including transport reactions between the compartments and the extracellular medium. The reaction list consists of 872 internal metabolites involved in a total of 1220 reactions, whereof 473 relate to known open reading frames. Initial in silico analysis of the reconstructed model is presented.