Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence
Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence
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DOI：
10.1016/s0168-9452(00)00373-3
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发表时间：
2000-12-07
期刊：
影响因子：
5.2
通讯作者：
Ueda, T
中科院分区：
文献类型：
--
作者：
Fukuchi-Mizutani, M;Ishiguro, K;Ueda, T
以番茄脂肪氧合酶基因片段为探针，从衰老玫瑰花瓣的cDNA文库中克隆到一个编码脂肪氧合酶基因Rlox1的基因。在大肠杆菌中表达的Rlox1蛋白的鉴定表明，Rlox1蛋白是一种可溶性脂肪氧合酶，在酸性区域(pH 4.5-5.0)具有不寻常的最适pH。Northern印迹分析表明，Rlos1基因的转录水平随着月季花瓣的衰老而显著增加。用乙烯处理月季花也提高了Rlox1基因的mRNA。这些结果表明，Rloxl脂肪氧合酶参与了月季花的衰老。(C)2000爱思唯尔爱尔兰科学有限公司。保留所有权利。
A cDNA encoding lipoxygenase, Rlox1, was isolated from a cDNA library of senescing rose petals using tomato lipoxygenase cDNA fragments as probes. Characterization of the Rlox1 protein expressed in Echerichia coli revealed that the Rlox1 protein was a soluble lipoxygenase with an unusual optimal pH in the acidic region (pH 4.5-5.0). Northern blot analysis showed that the transcript of the Rlos1 gene was dramatically increased in response to senescence of rose petals. Treatment of rose flowers with ethylene also elevated the mRNA of the Rlox1 gene. These results suggest that the Rloxl lipoxygenase is involved in senescence of rose flowers. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.