FORMATION OF THE 3' END OF U1 SNRNA IS DIRECTED BY A CONSERVED SEQUENCE LOCATED DOWNSTREAM OF THE CODING REGION
FORMATION OF THE 3' END OF U1 SNRNA IS DIRECTED BY A CONSERVED SEQUENCE LOCATED DOWNSTREAM OF THE CODING REGION
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DOI：
10.1002/j.1460-2075.1985.tb03857.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
HERNANDEZ, N
中科院分区：
文献类型：
--
作者：
HERNANDEZ, N
U1是一种小的非多聚腺苷酸化的核RNA，由RNA聚合酶II转录，已知在mRNA剪接中发挥作用。U1 snRNA的成熟3“末端至少在4个步骤中形成。第一步产生U1 RNA的前体，在3“末端有一些额外的核苷酸;在第二步中，这些前体被缩短为成熟的U1 RNA。确定了第1步所需的序列。构建了在编码区3“端附近有各种缺失和取代的人U1基因，并通过DNA转染将其导入HeLa细胞。通过S1作图分析了外源U1基因瞬时表达过程中合成的RNA的结构。位于U1编码区下游并在不同物种的U1、U2和U3基因中保守的13个核苷酸序列是指导U1 snRNA 3“端形成的第一步所需的唯一序列。
U1 is a small non-polyadenylated nuclear RNA that is transcribed by RNA polymerase II and is known to play a role in mRNA splicing. The mature 3'' end of U1 snRNA is formed in at least 4 steps. The 1st step generates precursors of U1 RNA with a few extra nucleotides at the 3'' end; in the 2nd step, these precursors are shortened to mature U1 RNA. The sequences required for the 1st step were determined. Human U1 genes with various deletions and substitutions near the 3'' end of the coding region were constructed and introduced into HeLa cells by DNA transfection. The structure of the RNA synthesized during transient expression of the exogenous U1 gene was analyzed by S1 mapping. A 13 nucleotide sequence located downstream from the U1 coding region and conserved among U1, U2 and U3 genes of different species is the only sequence required to direct the 1st step in the formation of the 3'' end of U1 snRNA.