Platination of the siRNA sense-strand modulates both efficacy and selectivity in vitro
Platination of the siRNA sense-strand modulates both efficacy and selectivity in vitro
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DOI：
10.1016/j.bbrc.2007.06.131
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发表时间：
2007-09-14
影响因子：
3.1
通讯作者：
Elmroth, Sofi K. C.
中科院分区：
文献类型：
--
作者：
Hagerlof, Margareta;Hedman, Hanna;Elmroth, Sofi K. C.
使用短干扰RNA（siRNA）选择性抑制蛋白质产生已迅速成为瞬时调节蛋白质水平的常用技术。在本论文中，我们研究了在有义链中引入铂化碱基是否可以用于调节siRNA的功效。研究了四种不同的siRNA，所有siRNA都靶向Wnt-5a mRNA的初始富含AU的3' UTR。使用荧光素酶作为报告基因，在体外对siRNA的解链特性和翻译抑制作用进行表征。翻译抑制研究表明，所有铂化siRNA仍然有效。对于与荧光素酶基因具有部分互补性的siRNA，铂化显示减少脱靶效应。发现所有siRNA在细胞体外翻译系统中均具有活性，对于所研究的大多数siRNA，抑制水平远高于80%。(c)2007年爱思唯尔公司All rights reserved.
The use of short interfering RNAs (siRNA) for selective suppression of protein production has rapidly become a commonly used technique for transient modulation of protein levels. In the present paper, we investigate whether introduction of platinated bases in the sense strand can be used to modulate the efficacy of siRNAs. Four different siRNAs were studied, all targeting the initial AU-rich 3' UTR of Wnt-5a mRNA. The siRNAs were characterized with respect to melting properties and translational inhibitory effect in vitro using luciferase as a reporter gene. The translation inhibition studies reveal that all platinated siRNA remain efficient. For an siRNA with partial complementarity to the lucifierase gene, platination was shown to reduce the off-target effects. All siRNAs were found to be active in cellular in vitro translation systems, reaching suppression levels well above 80% for the majority of siRNAs investigated. (c) 2007 Elsevier Inc. All rights reserved.