Real time imaging of single fluorophores on moving actin with an epifluorescence microscope.
Real time imaging of single fluorophores on moving actin with an epifluorescence microscope.
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使用落射荧光显微镜对移动肌动蛋白上的单个荧光团进行实时成像。
DOI：
10.1016/s0006-3495(95)79937-4
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发表时间：
1995
影响因子：
3.4
通讯作者：
K. Kinosita
中科院分区：
文献类型：
--
作者：
I. Sase;H. Miyata;J. Corrie;J. S. Craik;K. Kinosita
对普通荧光显微镜进行相对简单的改进，大大降低了其背景发光，允许在水介质中连续和实时地成像单个荧光团。主要的修改是改变激发光路和设置光圈光圈，以使杂散光不会在显微镜内散射。提出了一种使用肌动蛋白细丝来确定观察到的荧光团奇异性的简单而准确的方法。在30帧/S的视频速率下，可以成像结合到肌动蛋白细丝上的单个四甲基罗丹明荧光团，滑动到沉重的肌球蛋白上。对运动荧光分子的成功成像表明，常规显微镜可能成为研究生理环境中单个生物分子之间动态相互作用的常规工具。
Relatively simple modifications of an ordinary epifluorescence microscope have greatly reduced its background luminescence, allowing continuous and real time imaging of single fluorophores in an aqueous medium. Main modifications were changing the excitation light path and setting an aperture stop so that stray light does not scatter inside the microscope. A simple and accurate method using actin filaments is presented to establish the singularity of the observed fluorophores. It was possible, at the video rate of 30 frames/s, to image individual tetramethylrhodamine fluorophores bound to actin filaments sliding over heavy meromyosin. The successful imaging of moving fluorophores demonstrates that conventional microscopes may become a routine tool for studying dynamic interactions among individual biomolecules in physiological environments.
聚合诱导的用碘乙酰胺四甲基罗丹明标记的肌动蛋白的荧光变化。
影响因子：
3.9
作者：
Tait,JF;Frieden,C
通讯作者：
Frieden,C
DOI：
10.1073/pnas.83.17.6272
发表时间：
1986-09-01
影响因子：
11.1
作者：
KRON, SJ;SPUDICH, JA
通讯作者：
SPUDICH, JA