Expression of metalloproteinases and metalloproteinase inhibitor in human arthritic synovium.
Expression of metalloproteinases and metalloproteinase inhibitor in human arthritic synovium.
复制标题
金属蛋白酶和金属蛋白酶抑制剂在人关节炎滑膜中的表达。
DOI：
10.1002/art.1780340904
复制
发表时间：
1991
影响因子：
--
通讯作者：
McCachren,SS
中科院分区：
文献类型：
--
作者：
McCachren,SS
应用RNA探针原位检测中性金属蛋白酶信使RNA及其组织抑制物(TIMP)在人关节炎滑膜中的表达。活动期类风湿关节炎(RA)患者滑膜衬里细胞表达间质胶原酶和基质分解素。在表达单核巨噬细胞抗原的细胞和表达单核巨噬细胞抗原的阴性细胞中都发现了蛋白水解酶信使RNA。TIMP也主要沿滑膜衬里层表达。在高度炎症的RA中，TIMP的表达似乎没有蛋白水解酶那么强。在骨性关节炎滑膜中，TIMP的表达水平较高，而胶原酶和基质分解酶的表达较弱。在炎症过程中，滑膜中金属蛋白酶和金属蛋白酶抑制物的表达之间的平衡似乎发生了改变。这些结果与滑膜在RA和骨性关节炎的软骨损伤中起不同作用的观点是一致的。
The expression of messenger RNA encoding neutral metalloproteinases and the tissue inhibitor of metalloproteinases (TIMP) in human arthritic synovium was evaluated in situ, using RNA probes. Interstitial collagenase and stromelysin were expressed by synovial lining cells in patients with active rheumatoid arthritis (RA). Proteinase messenger RNA was found both in cells expressing mononuclear phagocyte antigens and in cells that were negative for the antigens. TIMP was also expressed predominantly along the synovial lining layer. In highly inflammatory RA, TIMP expression appeared less intense than that of the proteases. In osteoarthritic synovium, TIMP was expressed at easily detectable levels, whereas the expression of collagenase and stromelysin was less prominent. The balance between expression of the metalloproteinases and of the metalloproteinase inhibitor in synovium appears to be altered during inflammation. These results are consistent with the notion that synovium plays different roles in the cartilage damage of RA and of osteoarthritis.