Selective secretion of chemoattractants for haemopoietic progenitor cells by bone marrow endothelial cells: a possible role in homing of haemopoietic progenitor cells to bone marrow
Selective secretion of chemoattractants for haemopoietic progenitor cells by bone marrow endothelial cells: a possible role in homing of haemopoietic progenitor cells to bone marrow
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DOI：
10.1046/j.1365-2141.1999.01644.x
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发表时间：
1999-09-01
影响因子：
6.5
通讯作者：
Hosokawa, M
中科院分区：
文献类型：
--
作者：
Imai, K;Kobayashi, M;Hosokawa, M
为了阐明造血祖细胞在骨髓中的机制，我们研究了骨髓和肺内皮细胞对造血祖细胞的趋化因子的分泌。骨髓内皮细胞，但不是肺内皮细胞，分泌造血祖细胞系，FDCP-2，和正常的造血祖细胞的趋化因子。生物标记板分析表明，骨髓内皮细胞条件培养液具有趋化活性和随机运动刺激活性。骨髓内皮细胞表达基质细胞衍生因子-1（SDF-1）mRNA并产生SDF-1蛋白，而肺内皮细胞则不表达。抗SDF-1抗体可部分抑制FDCP-2细胞与骨髓内皮细胞的粘附。这些结果表明，造血祖细胞的趋化因子，包括SDF-1和随机运动刺激因子（S）选择性地分泌的骨髓内皮细胞可能有助于造血祖细胞归巢到骨髓。
To elucidate the mechanisms by which haemopoietic progenitor cells lodge in the bone marrow, we examined the secretion of chemoattractants for haemopoietic progenitor cells by bone marrow and lung endothelial cells. The bone marrow endothelial cells, but not lung endothelial cells, secreted chemoattractants for the haemopoietic progenitor cell line, FDCP-2, and normal haemopoietic progenitor cells. Checkerboard analysis demonstrated that the conditioned medium of the bone marrow endothelial cells had chemotactic activity and random motility-stimulating activity. The bone marrow endothelial cells expressed stromal-cell-derived factor-1 (SDF-1) mRNA and produced SDF-1 protein, whereas the lung endothelial cells did not. Adhesion of FDCP-2 cells to the bone marrow endothelial cells was partially inhibited by anti-SDF-1 antibody. These findings suggest that the chemoattractants for haemopoietic progenitor cells including SDF-1 and random motility-stimulating factor(s) selectively secreted by the bone marrow endothelial cells may contribute to the homing of haemopoietic progenitor cells to bone marrow.