Clonal relatedness and resistance characteristics of OXA-24 and-58 producing carbapenem-resistant Acinetobacter baumannii isolates in Tehran, Iran
Clonal relatedness and resistance characteristics of OXA-24 and-58 producing carbapenem-resistant Acinetobacter baumannii isolates in Tehran, Iran
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DOI：
10.1111/jam.14409
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发表时间：
2019-09-10
影响因子：
4
通讯作者：
Kadkhoda, H.
中科院分区：
文献类型：
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作者：
Salehi, B.;Ghalavand, Z.;Kadkhoda, H.
目的对伊朗德黑兰附近两家医院的耐碳青霉烯鲍曼不动杆菌（Acinetobacter baumannii， CRAB）的耐碳青霉烯耐药性机制及分子流行病学进行研究。方法与结果对180株螃蟹分离株进行了研究。药敏试验采用纸片扩散法和Epsilometer法。所有分离株采用聚合酶链反应检测OXA-23、-24和-58。随后，研究了含有OXA-24和-58的分离株Ambler A类金属β -内酰胺酶（MBLs）和碳青霉烯水解D类β -内酰胺酶（ISAba1）的抗性决定因素，并利用脉冲场凝胶电泳（PFGE）分析了它们之间的遗传相关性。所有分离株均对亚胺培南耐药，MIC为8 μ g ml(-1)，对粘菌素敏感，MIC为
Aims The present study was conducted to investigate the mechanism of carbapenem resistance and the molecular epidemiology of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates collected from two nearby hospitals in Tehran, Iran. Methods and Results A total of 180 CRAB isolates were studied. Antimicrobial susceptibility testing was performed using disk diffusion and Epsilometer tests. The detection of OXA-23, -24 and -58 was implemented for all isolates using polymerase chain reaction. Subsequently, isolates harbouring OXA-24 and -58 were investigated for the presence of resistance determinants of Ambler class A, metallo-beta-lactamases (MBLs), and carbapenem-hydrolysing class D beta-lactamases, ISAba1, and the genetic relatedness between them was analysed using pulsed-field gel electrophoresis (PFGE). All isolates were found to be resistant to imipenem with a MIC of >= 8 mu g ml(-1) and were susceptible to colistin with a MIC of