Morphological changes of retinal pigment epithelium and choroid in rd-mice
Morphological changes of retinal pigment epithelium and choroid in rd-mice
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DOI：
10.1006/exer.1998.0589
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发表时间：
1999-01-01
影响因子：
3.4
通讯作者：
Lütjen-Drecoll, E
中科院分区：
文献类型：
--
作者：
Neuhardt, T;May, CA;Lütjen-Drecoll, E
研究了视网膜变性(RD)小鼠视网膜色素上皮(RPE)和脉络膜退行性改变的顺序与光感受器改变的关系。对3周龄至26月龄的动物进行了光镜、透射电子显微镜、酶组织化学和定量形态观察。此外，还用腐蚀铸型标本的扫描电子显微镜观察了脉络膜毛细血管(CC)的变化。在3周龄的小鼠中，视网膜中央部分的大部分光感受器外段已经消失，但仍有细胞残留，RPE增大，并显示细长的微绒毛。在8周龄的动物中，光感受器在后极区的大片区域完全缺失。在这些地区，RPE也完全消失了。组织学连续切片的定量评估表明，RPE的丢失以无RPE的Bruch膜长度为指标，一直持续增加到20月龄。在8周龄的动物中，退变的RPE附近的CC显示窗口消失。在10周龄的动物中，CC消失在RPE已经缺乏的区域。CC的丢失随着年龄的增长而增加，20月龄动物的CC缺失率为5-10%。相关小动脉和烟酰胺腺嘌呤二核苷酸磷酸黄递酶(NADPH-d)阳性神经纤维的丢失仅发生在类似2岁的RD小鼠。与其他动物模型相比，RD小鼠的RPE和CC缺陷相对较大。因此，RD小鼠可能为研究参与CC退变的因素提供一个很好的工具。(C)1999年学术出版社。
The sequence of degenerative changes in the retinal pigment epithelium (RPE) and the choroid of retinal degeneration (rd)-mice was studied in correlation with photoreceptor changes. Three weeks to 26-month-old animals were investigated using light and transmission electron microscopy, enzyme histochemistry and quantitative morphology. Changes in the choriocapillaris (CC) were additionally studied by scanning electron microscopy of corrosion cast preparations. In 3-week-old mice, in which most of the outer segments of photoreceptors in the central portion of the retina had disappeared but remnants of the cells were still present, the RPE was enlarged and showed elongated microvilli. In 8-week-old animals, the photoreceptors were completely absent in large areas of the posterior pole region. In these areas the RPE was also completely lost. Quantitative evaluation performed in histological serial sections showed that loss of RPE measured as length of RPE-free Bruch's membrane, continuously increased up to the age of 20 months. In 8-week-old animals, CC adjacent to degenerating RPE showed loss of fenestration. In 10-week-old animals, the CC disappeared in those areas where the RPE was already lacking. The loss of CC increased with increasing age and in 20-month-old animals 5-10 % of the entire CC was lacking. Loss of the related arterioles and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d)-positive nerve fibers occurred only in similar to 2-year-old rd-mice. Compared to other animal models, RPE and CC defects in rd-mice are relatively large. The rd-mice might therefore provide a good tool to study factors involved in CC degeneration. (C) 1999 Academic Press.