Differences between betaproteobacterial ammonia-oxidizing communities in marine sediments and those in overlying water

Differences between betaproteobacterial ammonia-oxidizing communities in marine sediments and those in overlying water
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DOI:
10.1128/aem.70.6.3789-3793.2004
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发表时间:
2004-06-01
影响因子:
4.4
通讯作者:
Prosser, JI
Prosser, JI
中科院分区:
生物学2区
文献类型:
--
作者:
Freitag, TE;Prosser, JI

文献摘要

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为了评估betaproteobacterial氨氧化细菌(AOB)在海洋沉积物和上覆水,社区在洛赫,苏格兰,其特征在于克隆文库和16 S rRNA基因片段的变性梯度凝胶电泳分析之间的联系。亚硝化螺菌群集I类序列分离出两种环境,但不同的序列类型占主导地位的水和沉积物样品。详细的系统发育分析海洋亚硝化螺菌集群1-样序列在洛赫杜伊希和周边地区的存在,至少有两个不同的系统发育亚群,潜在的指示内betaproteobacterial AOB,代表不同的海洋生态类型的新的谱系。
To assess links between betaproteobacterial ammonia-oxidizing bacteria (AOB) in marine sediment and in overlying water, communities in Loch Duich, Scotland, were characterized by analysis of clone libraries and denaturant gradient gel electrophoresis of 16S rRNA gene fragments. Nitrosospira cluster I-like sequences were isolated from both environments, but different sequence types dominated water and sediment samples. Detailed phylogenetic analysis of marine Nitrosospira cluster 1-like sequences in Loch Duich and surrounding regions suggests the existence of at least two different phylogenetic subgroups, potentially indicative of new lineages within the betaproteobacterial AOB, representing different marine ecotypes.