Chronic toxicity of both raw and processed Polygoni Multiflori Radix on rats
Chronic toxicity of both raw and processed Polygoni Multiflori Radix on rats
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生何首乌炮制品对大鼠的慢性毒性
DOI：
10.5246/jcps.2016.01.006
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发表时间：
2016
影响因子：
--
通讯作者：
Ronghua Zhao
中科院分区：
文献类型：
--
作者：
Yanran He;Meizhen Song;Wangen Wang;Pei Lin;Yunfei Li;Wen Gu;Jie Yu;Ronghua Zhao
近年来，黄芪和熟地黄的毒副作用引起了国内外的广泛关注。这些不良反应多发生在大剂量、疗程延长的病例中。肝脏通常是这些不良反应的靶器官。本研究采用体内慢性毒性试验方法，探讨了中药PMR和PMRP水提物主要成分和总蒽醌与慢性毒性的关系。雌雄SD大鼠分别灌胃PMR和PMRP水提液及总蒽醌12周。我们评估了基本的生化指标，进行了主要器官的显微镜观察，并评估了肝和肾纤维化的早期指标。辛伐他汀主要治疗高胆固醇血症和高甘油三酯血症。并对毒副作用关系进行了讨论。在我们的研究中，五只雄性大鼠死亡，而所有雌性大鼠都存活下来，这表明可能涉及一些性别差异。在研究过程中，体重发生了显著变化，基础生化指标也有零星发生。12周后肝、肾组织病理学改变轻微，无剂量依赖关系。几乎所有雄性大鼠均观察到血清层粘连蛋白（LN）升高，提示肝、肾纤维化风险仍然存在，尤其是雄性大鼠，尽管肝、肾病理检查未发现纤维化。PMR和PMRP给药12周后，未观察到重大和严重的不良反应。用药期间仍需进行定期安全监测，以预防可能的风险。关键词：慢性毒性，黄芪，制何首乌，总蒽醌。
Recently, adverse effects of Polygoni Multiflori Radix (PMR) and Polygoni Multiflori Radix Praeparata (PMRP) have attracted intensive attention worldwide. These adverse effects most occurred in cases with high dose of prolonged medication course. Liver is usually the target organ of these adverse effects. In the present research, we performed in vivo chronic toxicity study and aimed to evaluate relationships between major constituents of water extractions and total anthraquinone of PMR and PMRP and chronic toxicity. SD rats of both sexes were given water extractions as well as total anthraquinone of PMR and PMRP for 12 weeks. We evaluated basic biochemical indexes, conducted microscopic observations of main organs and assessed early indicators of liver and renal fibrosis. Simvastatin, with hypertriglyceridemia and hypercholesterolemia as its main therapeutic areas, was investigated in our study. Component-toxicity relationships were also discussed. Five male rats died in our study, while all female rats survived, suggesting that some gender differences might be involved. Body weight was significantly changed, and basic biochemical indexes were sporadically occurred during the research. Pathological examinations on liver and kidney showed slight alternations after 12 weeks without dose-dependent relationship. Increase in serum laminin (LN) was observed in almost all male rats, indicating that the risks of liver or kidney fibrosis still existed, especially for males, although no fibrosis wa s found in the pathological examination of liver and kidney. No major and severe adverse effects were observed after 12 weeks of administration of PMR and PMRP. Regular safety monitoring is still necessary during medication in order to prevent possible risks. Keywords: Chronic toxicity, Polygoni Multiflori Radix, Polygoni Multiflori Radix Praeparata, Total anthraquinone.