Baicalein exerts neuroprotective effects in 6-hydroxydopamine-induced experimental parkinsonism in vivo and in vitro
Baicalein exerts neuroprotective effects in 6-hydroxydopamine-induced experimental parkinsonism in vivo and in vitro
复制标题
黄芩素在 6-羟基多巴胺诱导的体内和体外实验性帕金森病中发挥神经保护作用
DOI：
10.1016/j.pbb.2009.03.008
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发表时间：
2009-06-01
影响因子：
3.6
通讯作者：
Du, Guanhua
中科院分区：
文献类型：
--
作者：
Mu, Xin;He, Guorong;Du, Guanhua
黄芩素是从中药黄芩根中提取的黄酮类化合物，已被证明对神经元发挥保护作用，防止多种神经元损伤。本研究的目的是探讨黄芩素对 6-羟基多巴胺 (6-OHDA) 诱导的体外和体内实验性帕金森症的神经保护作用。在体外实验中，我们发现黄芩素（0.5、5μg/mL）可以显着改善6-OHDA诱导的SH-SY5Y细胞凋亡，从6-OHDA组的31.56%改善到18.90%。分别为21.61%。并促进PC12细胞的神经突生长。在体内实验中，黄芩素对阿朴吗啡（APO）诱导的旋转没有影响，但它可以显着减弱6-OHDA损伤大鼠的肌肉震颤。与6-OHDA组相比，突发频率和幅度分别为13.43%、35.18%。此外，黄芩素治疗还可以将6-OHDA组的酪氨酸羟化酶（TH）阳性神经元增加至265.52%。黄芩素的神经保护作用与黑质内星形胶质细胞反应的减弱相一致。通过增加多巴胺能神经元数量证明黄芩素的神经保护作用可能部分是由黄芩素的抗凋亡、促分化和抗炎机制引起的。因此，黄芩素由于其抗凋亡、促分化和抗炎作用，可能成为预防或治疗帕金森病的有希望的候选者。 (c) 2009 Elsevier Inc. All Fights 保留。
Baicalein, a flavonoid obtained from the root of Chinese medicinal herb Scutellaria baicalensis, has been shown to exert a protective effect on neurons against several neuronal insults. The aim of this study was to explore the neuroprotective effect of baicalein in 6-hydroxydopamine (6-OHDA)-induced experimental parkinsonism in vitro and in vivo. In in vitro experiments, we found that baicalein (0.5, 5 mu g/mL) could significantly ameliorate the 6-OHDA-induced SH-SY5Y cell apoptosis from 31.56% in the 6-OHDA group to 18.90%. 21.61% respectively. and also promote neurite outgrowth of PC12 cell. In in vivo experiments, baicalein had no effect on apomorphine (APO)-induced rotations, but it could significantly attenuate muscle tremor of 6-OHDA-lesioned rats. The burst frequency and amplitude are 13.43%, 35.18% compared to 6-OHDA group. Moreover, baicalein treatment could also increase tyrosine hydroxylase (TH)-positive neurons to 265.52% of the 6-OHDA group. The neuroprotective action of baicalein was coincident with an attenuated astroglial response within the substantia nigra. Neuroprotective effect of baicalein as demonstrated by the increasing the number of dopaminergic neurons may have been, in Part, caused by anti-apoptotic, pro-differentiation and anti-inflammatory mechanisms of baicalein. Therefore, baicalein can be a promising candidate for prevention or treatment of Parkinson's disease, owing to its anti-apoptotic, pro-differentiation and anti-inflammatory action. (c) 2009 Elsevier Inc. All Fights reserved.