An ultrasensitive aptasensor for Ochratoxin A using hexagonal core/shell upconversion nanoparticles as luminophores
An ultrasensitive aptasensor for Ochratoxin A using hexagonal core/shell upconversion nanoparticles as luminophores
复制标题
使用六角核/壳上转换纳米粒子作为发光团的赭曲霉毒素 A 超灵敏适体传感器
DOI：
10.1016/j.bios.2017.01.009
复制
发表时间：
2017-05-15
影响因子：
12.6
通讯作者：
Wang, Zhouping
中科院分区：
文献类型：
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作者：
Dai, Shaoliang;Wu, Shijia;Wang, Zhouping
我们利用核/壳上转换纳米粒子（CS-UCNPs）作为发光团，开发了一种用于赭曲毒素A （OTA）检测的超灵敏发光共振能量转移（LRET）适体传感器。将OTA适配体作为能量供体标记在CS-UCNPs上，氧化石墨烯（GO）作为能量受体。适体与氧化石墨烯之间的π - π堆叠相互作用使CS-UCNPs与氧化石墨烯接近，从而启动LRET过程，导致CS-UCNPs发光猝灭。发光强度与OTA浓度的对数在0.001 ngmL(-1) ~ 250 ngmL（-1）范围内线性校准，检出限为0.001 ngmL（-1）。该传感器对啤酒样品中的OTA具有良好的特异性。该传感器在啤酒样品基体中的超高灵敏度和显著的鲁棒性为其在实际应用中提供了良好的前景。
We developed an ultrasensitive luminescence resonance energy transfer (LRET) aptasensor for Ochratoxin A (OTA) detection, using core/shell upconversion nanoparticles (CS-UCNPs) as luminophores. The OTA aptamer was tagged to CS-UCNPs as energy donor and graphene oxide (GO) acted as energy acceptor. The pi-pi stacking interaction between the aptamer and GO brought CS-UCNPs and GO in close proximity hence initiated the LRET process resulting in quenching of CS-UCNPs luminescence. A linear calibration was obtained between the luminescence intensity and the logarithm of OTA concentration in the range from 0.001 ng mL(-1) to 250 ng mL(-1), with a detection limit of 0.001 ngmL(-1). The aptasensor showed good specificity towards OTA in beer samples. The ultrahigh sensitivity and pronounced robustness in beer sample matrix suggested promising prospect of the aptasensor inpractical applications.