Glomerular permeability and polyanion in adriamycin nephrosis in the rat.
Glomerular permeability and polyanion in adriamycin nephrosis in the rat.
复制标题
大鼠阿霉素肾病的肾小球通透性和聚阴离子。
DOI：
10.1038/ki.1983.139
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发表时间：
1983
影响因子：
19.6
通讯作者：
H. Rennke
中科院分区：
文献类型：
--
作者：
J. J. Weening;H. Rennke;H. Rennke
通过测量实验动物和对照动物中不同分子半径（ae）的不带电标记葡聚糖和阴离子、天然和阳离子辣根过氧化物酶（HRP）的清除率分数（C/GFR），研究了阿霉素诱导的大鼠蛋白尿中肾小球通透性的变化。在单次静脉注射阿霉素（阿霉素）7.5 mg/kg后的第14天和第55天之间对实验动物进行研究。在实验动物的平均蛋白尿为98毫克/24小时。肾小球形态显示，除了上皮细胞肿胀，空泡化，足突闭塞，肾小球胶体铁染色显着减少很少的变化。聚乙烯亚胺染色显示，与对照组相比，蛋白尿大鼠的肾内板和肾外板中阴离子位点的分布相似。菊糖清除率显示GFR和RPF分别降低20%和15%。右旋糖酐C/GFR值显示，实验动物中ae超过40 A的分子存在尺寸缺陷，与对照动物中ae为58和60 A的右旋糖酐的值相比增加了4 - 5倍。过氧化物酶清除率显示，实验动物中阴离子HRP的C/GFR略有增加，这可以根据筛分缺陷进行预期，而天然和阳离子HRP的C/GFR值几乎没有变化，表明蛋白尿动物中的功能性电荷屏障完整。（250字处删节）
Alterations in glomerular permeability were studied in Adriamycin-induced proteinuria in rats by measuring fractional clearances (C/GFR) of uncharged labeled dextrans of varying molecular radii (ae) and of anionic, native, and cationic horseradish peroxidases (HRP) in experimental and control animals. Experimental animals were studied between days 14 and 55 after a single intravenous dose of Adriamycin (doxorubicin), 7.5 mg/kg. Mean proteinuria in the experimental animals was 98 mg/24 hr. Glomerular morphology showed few changes except for epithelial cell swelling, vacuolization, and foot process obliteration, and a significant reduction of glomerular colloidal iron staining. Polyethyleneimine staining revealed a similar distribution of anionic sites in the laminae rarae interna and externa in proteinuric rats as compared with controls. Inulin clearances revealed reduction in GFR and RPF of 20 and 15%, respectively. Dextran C/GFR values showed in experimental animals a size defect for molecules with an ae exceeding 40 A, with a four- to fivefold increase over the values found in control animals for dextrans with ae of 58 and 60 A. The peroxidase clearances showed a slight increase in C/GFR of anionic HRP in experimental animals, as could be expected on the basis of the sieving defect, whereas the C/GFR values for native and cationic HRP were virtually unchanged, indicating an intact functional charge barrier in the proteinuric animals.(ABSTRACT TRUNCATED AT 250 WORDS)
大鼠氨基核苷肾病肾小球滤过器电荷和尺寸选择性屏障的变化。
DOI：
--
发表时间：
1981
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Olson,JL;Rennke,HG;Venkatachalam,MA
通讯作者：
Venkatachalam,MA
内脏上皮局部脱离导致肾小球通透性改变。
影响因子：
19.6
作者：
Kanwar,YS;Rosenzweig,LJ
通讯作者：
Rosenzweig,LJ