Downregulation of ultraspiracle gene expression delays pupal development in honeybees
Downregulation of ultraspiracle gene expression delays pupal development in honeybees
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DOI：
10.1016/j.jinsphys.2008.04.006
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发表时间：
2008-06-01
影响因子：
2.2
通讯作者：
Simoes, Zila L. P.
中科院分区：
文献类型：
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作者：
Barchuk, Angel Roberto;Figueiredo, Vera Lucia C.;Simoes, Zila L. P.
蜕皮类固醇与由核激素受体蛋白、蜕皮激素受体(ECR)和超阿司匹林(USE)组成的异二聚体结合后，调节昆虫生理的许多方面。一些证据表明，后者也在调节保幼激素(JH)的作用中发挥重要作用，从而整合了两种形态发生激素的信号。通过使用RNAi方法，我们在这里证明了us p参与了调节意大利蜜蜂蛹发育进程的机制，正如观察到的USP击倒蜜蜂的蛹发育延迟所表明的那样。基因敲除实验还表明，调控基因如FTZ转录因子1(FTZ-F1)和保幼激素酯酶(JHE)的表达依赖于USP。编码蜜蜂主要卵黄蛋白的基因卵黄蛋白原(VG)似乎不受USP的调控，这表明先前观察到的JH在幼虫发育的最后阶段诱导VG表达是由未知的转录因子复合体介导的。(C)2008爱思唯尔有限公司。保留所有权利。
Ecdysteroids regulate many aspects of insect physiology after binding to a heterodimer composed of the nuclear hormone receptor proteins ecdysone receptor (EcR) and ultraspiracle (Use). Several lines of evidence have suggested that the latter also plays important roles in mediating the action of juvenile hormone (JH) and, thus, integrates signaling by the two morphogenetic hormones. By using an RNAi approach, we show here that Us p participates in the mechanism that regulates the progression of pupal development in Apis mellifera, as indicated by the observed pupal developmental delay in usp knocked-down bees. Knock-down experiments also suggest that the expression of regulatory genes such as ftz transcription factor 1 (ftz-f1) and juvenile hormone esterase (jhe) depend on Usp. Vitellogenin (vg), the gene coding the main yolk protein in honeybees, does not seem to be under Usp regulation, thus suggesting that the previously observed induction of vg expression by JH during the last stages of pupal development is mediated by yet unknown transcription factor complexes. (C) 2008 Elsevier Ltd. All rights reserved.