Effects of cyclic adenosine 3',5'-monophosphate on chondrocyte terminal differentiation and cartilage-matrix calcification.
Effects of cyclic adenosine 3',5'-monophosphate on chondrocyte terminal differentiation and cartilage-matrix calcification.
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DOI：
10.1210/endo.137.1.8536602
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发表时间：
1996
期刊：
影响因子：
4.8
通讯作者：
Akitoshi Jikko;Hiroshi Murakami;Weiqun Yan;Kazuhisa Nakashima;Yoshie Ohya;Hisashi Satakeda;M. Noshiro;Takeshi Kawamoto;Shigeo Nakamura;Y. Okada;Fujio Suzuki;Yukio Kato
中科院分区：
文献类型：
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作者：
Akitoshi Jikko;Hiroshi Murakami;Weiqun Yan;Kazuhisa Nakashima;Yoshie Ohya;Hisashi Satakeda;M. Noshiro;Takeshi Kawamoto;Shigeo Nakamura;Y. Okada;Fujio Suzuki;Yukio Kato
我们研究了环磷酸腺苷对兔生长板软骨细胞终末分化和钙化的影响。二丁酰cAMP（dbcAMP），以及8-溴-cAMP取消软骨细胞大小，碱性磷酸酶活性，X型胶原合成，1 α，25-二羟维生素D3受体合成，45钙纳入不溶性材料，和钙含量的增加。所有这些都发生在肥大（终末）阶段的平行未处理培养物中。dbcAMP对碱性磷酸酶的抑制作用在24 h后可检测到，并且这种作用是可逆的。dbcAMP和8-溴环AMP在低浓度（3-5 μ M）下抑制碱性磷酸酶诱导和钙化，而刺激蛋白聚糖合成需要10-30倍的高浓度。这些结果表明，cAMP在抑制软骨细胞的终末分化和软骨基质钙化中起着至关重要的作用。
We examined the effects of cyclic AMP on terminal differentiation and calcification in rabbit growth plate chondrocyte cultures. Dibutyryl cAMP (dbcAMP), as well as 8-bromo-cAMP abolished the increases in chondrocyte size, alkaline phosphatase activity, type X collagen synthesis, 1 alpha, 25-dihydroxyvitamin D3 receptor synthesis, the incorporation of 45Ca into insoluble material, and the calcium content. All of these occurred in parallel untreated cultures during the hypertrophic (terminal) stage. The inhibition of alkaline phosphatase by dbcAMP was detectable after 24 h, and this effect was reversible. dbcAMP and 8-bromo-cyclic AMP inhibited alkaline phosphatase induction and calcification at low concentrations (3-5 microM), whereas 10-30-fold higher concentrations were required to stimulate proteoglycan synthesis. These findings suggest that cAMP plays a crucial role in suppressing terminal differentiation of chondrocyte and cartilage-matrix calcification.