Preservation of endothelial cells in excised rat carotid arteries. Effects of transmural pressure and segment length.
Preservation of endothelial cells in excised rat carotid arteries. Effects of transmural pressure and segment length.
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切除的大鼠颈动脉中内皮细胞的保存。
DOI：
10.1161/01.res.69.4.997
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发表时间：
1991
影响因子：
20.1
通讯作者：
Segal,SS
中科院分区：
文献类型：
--
作者：
Lewis,DA;Loomis,JL;Segal,SS
当动脉被切除时，它们会自发收缩和缩短，这可能会导致内皮受损。为了确定是否能在切除的血管中保留完整的内皮细胞层，我们分离并插管大鼠颈动脉两端的节段(原位长度，LIS，10-20 mm)，同时保持跨壁压力(70 Mm Hg)和LIS，同时跨壁压力或LIS，或允许血管塌陷和缩短(n=4条血管/治疗)。每次治疗后，血管节段注入固定剂，硝酸银染色显示内皮细胞。血管内皮细胞损失量通过对管腔表面的视频显微图像进行体视学分析来量化。结果表明，维持LIS和跨壁压力均可最大限度地减少内皮细胞的剥离(1.6+/-0.9%)；允许血管塌陷和缩短导致最大的内皮细胞损失(59.4+/-13.2%)；维持压力或LIS导致中等程度的内皮细胞损失(13-30%)。我们的结论是，通常伴随动脉节段切除的自发性塌陷和缩短会导致大量的内皮细胞丢失，这种情况可以通过维持跨壁压力和血管节段的长度来消除。这些发现对需要完整内皮细胞的外科和实验程序有一定的指导意义。
When arteries are excised, they collapse and shorten spontaneously, which can result in damage to the endothelium. To determine if an intact endothelial cell layer could be preserved in excised vessels, we isolated and cannulated rat carotid artery segments (in situ length, Lis, 10-20 mm) at both ends while maintaining both transmural pressure (70 mm Hg) and Lis, while either transmural pressure or Lis, or after allowing vessel collapse and shortening (n = 4 vessels per treatment). After each treatment, vessel segments were perfused with fixative and stained with AgNO3 to visualize endothelial cells. The amount of endothelial cell loss was quantified using stereological analysis of video microscopic images of the luminal surface. Results demonstrated that maintenance of both Lis and transmural pressure minimized endothelial cell denudation (1.6 +/- 0.9%); permitting the vessel to collapse and shorten resulted in the greatest loss of endothelial cells (59.4 +/- 13.2%); and maintenance of either pressure or Lis resulted in intermediate endothelial cell loss (13-30%). We conclude that the spontaneous collapse and shortening that normally accompany the excision of arterial segments result in substantial endothelial cell loss, which can be virtually eliminated with the maintenance of transmural pressure and vessel segment length. These findings have implications for surgical and experimental procedures requiring intact endothelium.
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血管内皮细胞连接的节段分化。
影响因子：
7.8
作者：
M. Simionescu;N. Simionescu;G. Palade
通讯作者：
G. Palade
由压力和缺血引起的主动脉冠状动脉搭桥术后静脉移植物的变化。
影响因子：
6
作者：
William R. Brody;Jon C. Kosek;William W. Angell
通讯作者：
William W. Angell
内皮源性舒张因子的生物测定：儿茶酚胺灭活。
DOI：
10.1152/ajpheart.1985.249.1.h95
发表时间：
1985
期刊：
The American journal of physiology
影响因子：
--
作者：
G. Rubanyi;R. Lorenz;P. Vanhoutte
通讯作者：
P. Vanhoutte
保留完整内皮的主动脉内膜中层制剂的形态和代谢。
DOI：
10.1172/jci108321
发表时间：
1976
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
ANrHoNY D. Morrison;Leonard BERwICK;L. Orci;Albert I. WNEmGRAD
通讯作者：
Albert I. WNEmGRAD
血管内皮细胞连接的节段分化。
影响因子：
7.8
作者：
M. Simionescu;N. Simionescu;G. Palade
通讯作者：
G. Palade