Systemic acute-phase reactants, C-reactive protein and haptoglobin, in adult periodontitis
Systemic acute-phase reactants, C-reactive protein and haptoglobin, in adult periodontitis
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DOI：
10.1111/j.1365-2249.1997.270-ce1162.x
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发表时间：
1997-02-01
影响因子：
4.6
通讯作者：
Willmann, DE
中科院分区：
文献类型：
--
作者：
Ebersole, JL;Machen, RL;Willmann, DE
应用生物素标记的单特异性抗体捕获ELISA法检测了成人牙周炎（AP）患者和正常人血清中的C-反应蛋白（CRP）和结合珠蛋白（Hp）。CRP为9.12 +/- 1.61 mg/l vs 2.17 +/- 0.41 mg/l（P < 0.001），Hp为3.68 +/- 0.37 g/l vs 1.12 +/- 0.78 g/l（P < 0.001）的AP患者血清中每种急性期反应物均显著升高。对牙周病患者临床特征的评估表明，在疾病活动性发作最频繁的患者中，CRP和Hp水平显著升高（分别为P < 0.02和P < 0.001）。纵向检查Hp水平显示洁治和根面平整后显著降低（3.68对2.38 g/l; P < 0.01）。50 mg/b.i.d.给药2年后，氟比洛芬（一种非甾体抗炎药）治疗1-2年后，Hp水平显著下降（P < 0.005），CRP水平下降35-40%（P < 0.05）。研究结果表明，局部感染导致牙周组织炎症增加和组织损失引起全身宿主变化，表现为两种急性期反应物的增加。结论是，这些分子要么在局部形成并分布到血清中，要么这些可能的局部感染影响宿主保护性反应的全身组分。
Capture ELISAs with biotinylated monospecific antibodies were developed to detect both C-reactive protein (CRP) and haptoglobin (Hp) in serum of adult periodontitis (AP) patients and normal subjects. Each acute-phase reactant was significantly increased in serum from AP patients with CRP at 9.12 +/- 1.61 mg/l versus 2.17 +/- 0.41 mg/l (P < 0.001) and Hp at 3.68 +/- 0.37 g/l versus 1.12 +/- 0.78 g/l (P < 0.001). Assessment of clinical characteristics of the patients' periodontal disease indicated that CRP and Hp levels were significantly increased in patients with the most frequent disease active episodes (P < 0.02 and P < 0.001, respectively). Longitudinal examination of the Hp levels showed a significant decrease following scaling and root planing (3.68 versus 2.38 g/l; P < 0.01). After a 2-year administration of 50 mg/b.i.d. Flurbiprofen (a non-steroidal anti-inflammatory drug), significantly decreased Hp levels were noted (P < 0.005), CRP levels declined by 35-40% after 1-2 years of treatment with the drug (P < 0.05). The findings indicated that localized infections resulting in increased inflammation and tissue loss in the periodontium elicit systemic host changes manifest by increases in two acute-phase reactants. The conclusions are that either these molecules are formed locally and distributed to the serum, or these presumably localized infections impact upon the systemic components of the host protective responses.