Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome.
Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome.
复制标题
肠易激综合征中类胰蛋白酶和蛋白酶激活受体 2 的表达水平
DOI：
10.5009/gnl14319
复制
发表时间：
2016-05-23
期刊：
影响因子：
3.4
通讯作者：
Zhang FC
中科院分区：
文献类型：
--
作者：
Liang WJ;Zhang G;Luo HS;Liang LX;Huang D;Zhang FC
背景/目的研究表明肥大细胞（MC）可激活蛋白酶激活受体并释放神经肽参与肠易激综合征（IBS）的发病。蛋白酶激活受体2（PAR-2）和类胰蛋白酶的水平有助于了解IBS的发病机制。方法对38例受试者（20例IBS腹泻[IBS-D]，8例IBS便秘[IBS-C]，10例健康志愿者）进行结肠镜活检。实时定量PCR和Western blot检测类胰蛋白酶和PAR-2的mRNA和蛋白水平。免疫组化法检测血管活性肠肽（VIP）、P物质（SP）、降钙素基因相关肽（CGRP）的含量，甲苯胺蓝染色法计数MC。结果IBS组类胰蛋白酶mRNA（p<0.05）、PAR-2 mRNA（p<0.05）和类胰蛋白酶蛋白水平（p <0.05）均显著高于正常对照组（p<0.05）。仅对于IBS-D观察到MC、CGRP、VIP和SP的升高（p<0.05，IBS-D相对于对照）。结论类胰蛋白酶可能通过上调PAR-2的功能，导致神经肽的释放，并与IBS的临床症状相关。
Background/Aims Previous studies have revealed that mast cells (MCs) may activate the protease-activated receptors and release of neuropeptides involved in the pathogenesis of irritable bowel syndrome (IBS). The levels of protease-activated receptor 2 (PAR-2) and tryptase can contribute to understanding the pathogenesis of IBS. Methods Colonoscopic biopsies were performed of 38 subjects (20 with IBS-diarrhea [IBS-D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue staining. Results Significant increases in the mRNA expression of tryptase (p<0.05, IBS-D, IBS-C vs control) and PAR-2 (p<0.05, IBS-D, IBS-C vs control) and in the tryptase protein level (p<0.05, IBS-D, IBS-C vs control) were detected in IBS. Elevations of MCs, CGRP, VIP and SP (p<0.05, IBS-D vs control) were observed for IBS-D only. Conclusions Tryptase levels may upregulate the function of PAR-2, resulting in the release of neuropeptide and they were correlated with clinical symptoms associated with IBS.