Treatment of chickpea with Rhizobium isolates enhances the expression of phenylpropanoid defense-related genes in response to infection by Fusarium oxysporum f. sp ciceris
Treatment of chickpea with Rhizobium isolates enhances the expression of phenylpropanoid defense-related genes in response to infection by Fusarium oxysporum f. sp ciceris
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DOI：
10.1016/j.plaphy.2007.04.004
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发表时间：
2007-06-01
影响因子：
6.5
通讯作者：
Cherif, M.
中科院分区：
文献类型：
--
作者：
Arfaoui, A.;El Hadrami, A.;Cherif, M.
使用 Northern 印迹分析研究了用根瘤菌分离株（PchDMS 和 Pch43）接种并进一步用尖孢镰刀菌 (Fusarium oxysporum f) 攻击的鹰嘴豆幼苗中参与苯丙素代谢的苯丙氨酸解氨酶 (PAL)、查尔酮合酶 (CHS) 和异黄酮还原酶 (IFR) 基因的差异表达。 sp。 ciceris (Foc) 种族 0。在接种 Foc 后的不同时间间隔，将中度抗性种质 INRAT87/1 中的基因激活模式与易感种质 ILC482 所表现出的基因激活模式进行比较，以确定转录物积累水平或时间的差异是否与鹰嘴豆种质对 Foc 的敏感性差异相关。中度抗性编号INRAT87/1中的基因激活高于易感ILC482。在接种病原体之前，用根瘤菌分离物对鹰嘴豆幼苗进行预处理，增强了三个基因 mRNA 转录本的积累。同时，分析了鹰嘴豆根中通过涉及这些酶的途径产生的可溶性酚池的变化。两种种质在 72 hpi 时均显示出这些化合物的强烈积累。此后，在中度抗性品种中，这些高水平的酚类化合物一直保持到实验结束，而在易感品种中，酚类化合物的含量显着下降。 HPLC 分析显示，与对照相比，在接种根瘤菌分离株和/或用 Foc 攻击的鹰嘴豆根中，组成型异黄酮、芒柄花素和生物鸡素 A 及其糖苷缀合物的积累量非常高。我们的结果表明，在接种 Foc 的鹰嘴豆幼苗中观察到酚类化合物积累的增加，可归因于苯丙素途径中基因表达的增加，并且用根瘤菌分离物预处理可增强此类基因表达。 (C) 2007 Elsevier Masson SAS。版权所有。
Differential expression of phenytalanine ammonia-lyase (PAL), chalcone synthase (CHS) and isoflavone reductase (IFR) genes involved in phenylpropanoids metabolism was investigated using Northern blot analyses in chickpea seedlings bacterized with Rhizobium isolates (PchDMS and Pch43) and further challenged with Fusarium oxysporum f. sp. ciceris (Foc) race 0. Gene activation patterns in the moderately resistant accession INRAT87/1 were compared with those exhibited by the susceptible accession ILC482 at various time intervals after inoculation with Foc, to determine whether differences in levels or timing of transcript accumulation could be correlated with differences in the susceptibility of chickpea accessions to Foc. Gene activation was higher in the moderately resistant accession INRAT87/1 than in the susceptible ILC482. Pre-treatment of chickpea seedlings with Rhizobium isolates before inoculation with the pathogen enhanced the accumulation of the three genes' mRNA transcripts. In parallel, changes in the soluble phenolic pool produced through pathways involving these enzymes were analyzed in chickpea roots. A strong accumulation of these compounds was revealed at 72 hpi in both accessions. After that time, these high levels of phenolic compounds were maintained until the end of the experiment in the moderately resistant accession, while they have significantly declined in the susceptible accession. HPLC analyses revealed a very high accumulation of the constitutive isoflavones, formononetin and biochanin A and their glycoside conjugates in chickpea roots inoculated with Rhizobium isolates and/or challenged with Foc, as compared to the controls. Our results suggest that the increased accumulation of phenolic compounds, observed in chickpea seedlings inoculated with Foc, can be attributed to increased expression of genes in the phenylpropanoid pathway and that such gene expression is enhanced by pre-treatment with Rhizobium isolates. (C) 2007 Elsevier Masson SAS. All rights reserved.