Electrospinning of polyhydroxyalkanoate fibrous scaffolds: effects on electrospinning parameters on structure and properties
Electrospinning of polyhydroxyalkanoate fibrous scaffolds: effects on electrospinning parameters on structure and properties
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DOI：
10.1080/09205063.2013.862400
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发表时间：
2014-01
期刊：
影响因子：
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通讯作者：
T. Volova;D. Goncharov;A. Sukovatyi;A. Shabanov;E. Nikolaeva;E. Shishatskaya
中科院分区：
文献类型：
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作者：
T. Volova;D. Goncharov;A. Sukovatyi;A. Shabanov;E. Nikolaeva;E. Shishatskaya
在这项研究中，采用静电纺丝法从不同化学成分的PHA中制备超细纤维：P(3HB)和共聚物：P(3HB-co-4HB)、P(3HB-co-3HV)和P(3HB-co-3HHx)。研究了影响超细纤维直径和性能的主要工艺参数（聚合物浓度、溶液进料速率、工作距离和施加电压）并评估了它们的效果。该研究揭示了生产高质量超细纤维的静电纺丝参数，并确定了应该改变哪些参数来定制产品的性能。这项研究首次比较了不同化学成分的 PHA 的生物和物理机械参数，这些参数取决于构成聚合物的单体比例和超细纤维取向。不同PHA制备的定向超细纤维的机械强度高于随机定向超细纤维的机械强度；尚未发现超细纤维取向对表面性能有显着影响。通过 PHA 静电纺丝生产的纤维支架对 NIH 3T3 小鼠成纤维细胞的附着、生长和活力没有任何不利影响，并且所有这些支架都适合组织工程应用。
In this study, electrospinning was used to prepare ultrafine fibers from PHAs with different chemical compositions: P(3HB) and copolymers: P(3HB-co-4HB), P(3HB-co-3HV), and P(3HB-co-3HHx). The main process parameters that influence ultrafine fiber diameter and properties (polymer concentration, solution feeding rate, working distance, and applied voltage) have been investigated and their effects evaluated. The study revealed electrospinning parameters for the production of high-quality ultrafine fibers and determined which parameters should be varied to tailor the properties of the products. This study is the first to compare biological and physical-mechanical parameters of PHAs with different chemical compositions as dependent upon the fractions of monomers constituting the polymers and ultrafine fiber orientation. Mechanical strength of aligned ultrafine fibers prepared from different PHAs is higher than that of randomly oriented ones; no significant effect of ultrafine fiber orientation on surface properties has been found. None of the fibrous scaffolds produced by electrospinning from PHAs had any adverse effects on attachment, growth, and viability of NIH 3T3 mouse fibroblast cells, and all of them were found to be suitable for tissue engineering applications.