NEW-YORK-CITY MEDIUM FOR ENHANCED RECOVERY OF MYCOPLASMA-PNEUMONIAE FROM CLINICAL SPECIMENS
NEW-YORK-CITY MEDIUM FOR ENHANCED RECOVERY OF MYCOPLASMA-PNEUMONIAE FROM CLINICAL SPECIMENS
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DOI：
10.1128/jcm.17.6.1077-1080.1983
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发表时间：
1983-01-01
影响因子：
9.4
通讯作者：
WEINER, LB
中科院分区：
文献类型：
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作者：
GRANATO, PA;POE, L;WEINER, LB
比较了改良纽约市（MNYC）培养基和不含亚甲蓝的PPLO培养基（PPLO琼脂）支持M.肺炎克雷伯氏菌。咽标本收集自1070名访问锡拉丘兹大学学生健康中心[纽约，美国]的大学生。在这些患者中，623人有呼吸道感染症状;其余447人无呼吸道疾病症状。将咽拭子接种到PPLO肉汤中;孵育3天和14天后，将这些肉汤在MNYC培养基和PPLO琼脂上传代培养; 222株（20.7%）M.在这些固体培养基上回收了肺炎链球菌，其中大多数分离株（196株）从有症状的患者中回收。所有分离株均能在MNYC培养基上生长; 5株分离株在PLLO琼脂上未能生长。所有M.从有症状的患者中回收的肺炎支原体在孵育1-5天内在MNYC培养基上检测到;在PPLO琼脂上检测到支原体生长之前需要孵育5-7天。在这些支原体分离株中，在孵育3天内和在PPLO琼脂上检测到任何支原体生长之前，在MNYC培养基上检测到> 86%。从无症状患者中分离的支原体也观察到类似的恢复时间模式。因此，MNYC培养基比PPLO琼脂更有利于M.在PPLO葡萄糖肉汤中盲法传代培养72小时后，从临床标本中分离出肺炎克雷伯氏菌。
Modified New York City (MNYC) medium and PPLO medium without methylene blue (PPLO agar) were compared for their ability to support the growth of M. pneumoniae from clinical specimens. Pharyngeal specimens were collected from 1070 college students who visited the Syracuse University Student Health Center [New York, USA]. Of these patients, 623 were symptomatic for respiratory infection; the remaining 447 were asymptomatic for respiratory illness. Throat swabs were inoculated into PPLO broths; these broths were subcultured onto MNYC medium and PPLO agar after 3 and 14 days of incubation; 222 (20.7%) clinical isolates of M. pneumoniae were recovered on these solid media, with the majority of the isolates (196) recovered from symptomatic patients. All isolates grew on MNYC medium; 5 isolates failed to grow on PLLO agar. All isolates of M. pneumoniae recovered from symptomatic patients were detected on MNYC medium within 1-5 days of incubation; 5-7 days of incubation were required before mycoplasmal growth was detected on PPLO agar. Of these mycoplasma isolates, > 86% were detected on MNYC medium within 3 days of incubation and before the detection of any mycoplasmal growth on PPLO agar. A similar pattern of recovery times was observed for mycoplasmas isolated from asymptomatic patients. Thus, MNYC medium is better than PPLO agar in supporting the rapid growth of M. pneumoniae from clinical specimens after 72 h blind subculture in PPLO glucose broth.