Conservation of complex knotting and slipknotting patterns in proteins
Conservation of complex knotting and slipknotting patterns in proteins
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DOI：
10.1073/pnas.1205918109
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发表时间：
2012-06-26
影响因子：
11.1
通讯作者：
Stasiak, Andrzej
中科院分区：
文献类型：
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作者：
Sulkowska, Joanna I.;Rawdon, Eric J.;Stasiak, Andrzej
在分析所有可用的蛋白质结构的结和滑结的存在下，我们检测到一个严格的保护复杂的打结模式内和几个蛋白质家族之间，尽管他们的大序列差异。由于导致打结的天然蛋白质结构的蛋白质折叠途径比那些导致具有相似大小和序列的未打结蛋白质的折叠途径更慢且效率更低，因此打结模式的严格保守性表明这些蛋白质中的结和滑结具有重要的生理作用。虽然对结的功能作用知之甚少，但最近的研究已经证明了结和滑结的蛋白质稳定能力。一些保守的打结模式发生在形成跨膜通道的蛋白质中，其中活结环似乎将形成通道的跨膜螺旋捆绑在一起。
While analyzing all available protein structures for the presence of knots and slipknots, we detected a strict conservation of complex knotting patterns within and between several protein families despite their large sequence divergence. Because protein folding pathways leading to knotted native protein structures are slower and less efficient than those leading to unknotted proteins with similar size and sequence, the strict conservation of the knotting patterns indicates an important physiological role of knots and slipknots in these proteins. Although little is known about the functional role of knots, recent studies have demonstrated a protein-stabilizing ability of knots and slipknots. Some of the conserved knotting patterns occur in proteins forming transmembrane channels where the slipknot loop seems to strap together the transmembrane helices forming the channel.