Exhaustive exercise, endurance training, and acid hydrolase activity in skeletal muscle.
Exhaustive exercise, endurance training, and acid hydrolase activity in skeletal muscle.
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力竭运动、耐力训练和骨骼肌酸水解酶活性。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
J. Rantamäki
中科院分区：
文献类型：
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作者：
V. Vihko;A. Salminen;J. Rantamäki
8种酸水解酶和2种能量代谢酶的活性从属于以下组之一的小鼠的主要红色（股外侧肌近端头、股内侧肌和股中间肌）和主要白色（股外侧肌远端头）骨骼肌匀浆中测定：1)久坐对照，从未训练或疲劳；2)筋疲力尽的对照组，在杀戮前5、10或20天在跑步机上跑完一次；3)训练小鼠，运动至死亡；4)精疲力竭的训练小鼠，在杀戮前5、10、20天运动至精疲力竭，在此期间不运动；5)去训练老鼠，在杀戮前5天、10天或20天停止训练。在未训练但未训练的动物中，剧烈运动导致5天后纤维坏死，β -葡糖苷酸酶、β - n -乙酰氨基葡萄糖酸酶、芳基硫酸酯酶、核糖核酸酶、脱氧核糖核酸酶、组织蛋白酶D和组织蛋白酶C活性显著增加，尤其是在红色肌纤维中。训练增加了两种肌肉类型的柠檬酸合成酶、β -葡萄糖醛酸酶和组织蛋白酶D的活性，以及红肌肉中β - n -乙酰氨基葡萄糖苷酶、芳基硫酸酯酶和组织蛋白酶C的活性。去训练的影响很小。穷尽性运动可引起致死性和明显的亚致死性纤维损伤，表现为5天后肌纤维溶酶体系统的激活。训练通过引起对剧烈运动的破坏性影响的明显抵抗来影响细胞内稳态。适度增加的水解酶活性可能反映耐力训练肌肉中增加的周转。
The activity of eight acid hydrolases and two energy metabolism enzymes were assayed from homogenates of predominantly red (proximal heads of m. vastus lateralis, m. vastus medialis, and m. vastus intermedius) and predominantly white (distal head of m. vastus lateralis) skeletal muscle of mice belonging to one of the following groups: 1) sedentary controls, never trained or exhausted; 2) exhausted controls, exhausted once by running on a treadmill 5, 10, or 20 days before killing; 3) trained mice, exercising until killed; 4) exhausted trained mice, exercising until exhausted 5, 10 or 20 days before killing, not exercising during that period; and 5) detrained mice, terminating training 5, 10, or 20 days before killing. In untrained but not in trained animals, exhaustive exercise caused, 5 days afterward, fiber necrosis and a marked increase in the activities of beta-glucuronidase, beta-N-acetylglucosaminidase, arylsulphatase, ribonuclease, deoxyribonuclease, cathepsin D, and cathepsin C, especially in red muscle fibers. Training increased the activities of citrate synthase, beta-glucuronidase, and cathepsin D in both muscle types and those of beta-N-acetylglucosaminidase, arylsulphatase, and cathepsin C in red muscle. Effects of detraining were minor. Exhaustive exercise causes lethal and evidently also sublethal fiber injuries manifesting themselves as an activation of the lysosomal system of muscle fibers 5 days later. Training affects cellular homeostasis by causing an apparent resistance to the damaging effects of exhaustive exercise. Moderately increased hydrolase activities may reflect increased turnover in endurance-trained muscles.