Corticotropin-releasing factor neurons innervate dopamine neurons in the periventricular hypothalamus of juvenile macaques. Synaptic evidence for a possible companion neurotransmitter.
Corticotropin-releasing factor neurons innervate dopamine neurons in the periventricular hypothalamus of juvenile macaques. Synaptic evidence for a possible companion neurotransmitter.
复制标题
促肾上腺皮质激素释放因子神经元支配幼年猕猴室周下丘脑的多巴胺神经元。
DOI：
10.1159/000125249
复制
发表时间：
1989
影响因子：
4.1
通讯作者：
Goldsmith,PC
中科院分区：
文献类型：
--
作者：
Thind,KK;Goldsmith,PC
促肾上腺皮质激素释放因子（CRF）和多巴胺（DA）是内分泌和自主神经应激反应的重要组成部分。CRF神经元在室周核（PV）和小细胞室旁核（pvPVN）的前部出现接近A14 DA神经元在这些相同的位置。由于CRF已被证明是一种兴奋性神经递质，可能的CRF与DA系统的相互作用进行了研究，使用双标记免疫细胞化学。通过PV和pvPVN的冠状振动切片从秋水仙素处理和未处理的幼年雌性食蟹猴中获得。他们依次免疫染色酪氨酸羟化酶（TH）（以确定DA神经元）与PAP和DAB，CRF使用15 nm胶体金。光镜下PV和PVN内TH和CRF免疫反应阳性细胞体的重合区域明显，但未观察到双染成分。电镜下，CRF轴突与TH树突或胞体之间常出现不对称性突触。对称的轴体突触有时出现在这些CRF/TH突触附近，而对称的轴轴突触是罕见的。我们的结论是CRF神经元传出突触激活A14 DA神经元在灵长类动物PV和PVPVN。平行CRF/DA对称突触也表明共存的一些相同的CRF神经元内的同伴递质。我们自己以前的工作和最近的独立研究表明，这种递质可能是GABA。因此，CRF神经元系统，这是已知的改变分泌的几种垂体激素，也可以通过下丘脑室周DA神经元介导其他反应的压力。
Corticotropin-releasing factor (CRF) and dopamine (DA) are important integrators of the endocrine and autonomic response to stress. CRF neurons in the anterior portions of the periventricular nucleus (PV) and parvocellular paraventricular nucleus (pvPVN) occur close to A14 DA neurons in these same locations. Since CRF has been shown to act as an excitatory neurotransmitter, possible CRF interactions with the DA system were investigated using double-label immunocytochemistry. Coronal vibratome sections through the PV and pvPVN were obtained from colchicine-treated and nontreated juvenile female cynomolgus macaques. They were sequentially immunostained for tyrosine hydroxylase (TH) (to identify DA neurons) with PAP and DAB, and for CRF using 15 nm colloidal gold. By light microscopy, areas of coincidence of TH- and CRF-immunoreactive cell bodies in the PV and pvPVN were obvious, but double-stained elements were not observed. By electron microscopy, asymmetrical synapses frequently occured between CRF axons and TH dendrites or somata. Symmetrical axosomatic synapses sometimes appeared adjacent to these CRF/TH synapses, while symmetrical axoaxonic synapses were rare. We conclude that CRF neuronal efferents synaptically activate A14 DA neurons in the primate PV and pvPVN. Parallel CRF/DA symmetrical synapses also suggest coexistence of a companion transmitter within some of these same CRF neurons. Our own previous work and recent independent studies indicate that this transmitter is probably GABA. Thus the CRF neuronal system, which is known to alter secretion of several pituitary hormones, may also act through hypothalamic periventricular DA neurons to mediate other responses to stress.