Optimization of β-carotene production by a newly isolated Serratia marcescens strain
Optimization of β-carotene production by a newly isolated Serratia marcescens strain
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DOI：
10.2225/vol15-issue6-fulltext-4
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发表时间：
2012-11-01
影响因子：
2.7
通讯作者：
Chen, Weiping
中科院分区：
文献类型：
--
作者：
Wang, Bohua;Lin, Liping;Chen, Weiping
beta-carotene is a commonly used food colorant. In this work, a novel beta-carotene producing strain, Serratia marcescens RB3, was isolated and identified by physiological and biochemical tests, as well as 16S rDNA sequence analysis. The production of beta-carotene by S. marcescens RB3 was identified through HPLC analysis. The cultivation conditions for beta-carotene production by S. marcescens RB3 were optimized as 2.0% lactose, 2.0% peptone, 0.3% beef extract, 1.0% NaCl supplemented with 0.05% Fe2+, pH 6.0 and 30 degrees C. Under the optimal conditions, the yield of beta-carotene achieved 2.45 mu g/mL.
