DNA interactions of cobalt(III) mixed-polypyridyl complexes containing asymmetric ligands.
DNA interactions of cobalt(III) mixed-polypyridyl complexes containing asymmetric ligands.
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DOI：
10.1016/j.jinorgbio.2004.04.003
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发表时间：
2004-06
影响因子：
3.9
通讯作者：
Xiang-li Wang;Hui Chao;Hong Li;Xian-Lan Hong;Yunjun Liu;L. Tan;L. Ji
中科院分区：
文献类型：
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作者：
Xiang-li Wang;Hui Chao;Hong Li;Xian-Lan Hong;Yunjun Liu;L. Tan;L. Ji
合成并表征了3-(吡啶-2-基)-5，6-二苯基-三嗪(Pdtb)、3-(pyridine-2-yl)-as-triazino[5，6-f]acenaphthylene(Pdta)和3-(pyridine-2-yl)-as-triazino[5，6-f]phenanthroline(Pdtp)三种新型不对称配体及其钴(III)配合物。用光谱、粘度、循环伏安法和电泳法研究了这三种配合物与小牛胸腺DNA(CT-DNA)的结合。实验结果表明，插层配体的大小和形状对配合物与CT-DNA的结合亲和力有显著影响。配合物2和3还被发现促进pBR322 DNA在照射后从超螺旋形式I裂解到开放的环形形式II。
Three novel asymmetric ligands, 3-(pyridine-2-yl)-5,6-diphenyl-as-triazine (pdtb), 3-(pyridine-2-yl)-as-triazino[5,6-f]acenaphthylene (pdta) and 3-(pyridine-2-yl)-as-triazino[5,6-f]phenanthroline (pdtp) and their cobalt(III) complexes have been synthesized and characterized. Binding of the three complexes with calf thymus DNA (CT-DNA) has been investigated by spectroscopic methods, viscosity, cyclic voltammetry, and electrophoresis measurements. The experimental results indicate that the size and shape of the intercalated ligand have a marked effect on the binding affinity of complexes to CT-DNA. Complexes 2 and 3 have also been found to promote cleavage of plasmid pBR322 DNA from the supercoiled form I to the open circular form II upon irradiation.