Silver nanoparticle-coated suture effectively reduces inflammation and improves mechanical strength at intestinal anastomosis in mice
Silver nanoparticle-coated suture effectively reduces inflammation and improves mechanical strength at intestinal anastomosis in mice
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DOI：
10.1016/j.jpedsurg.2013.12.012
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发表时间：
2014-04-01
影响因子：
2.4
通讯作者：
Wong, Kenneth K. Y.
中科院分区：
文献类型：
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作者：
Zhang, Shiwei;Liu, Xuelai;Wong, Kenneth K. Y.
背景：我们之前的研究表明，银纳米颗粒（AgNPs）具有抗炎特性。在本研究中，我们将AgNPs包被在可吸收缝合线表面，通过肠吻合模型进一步探讨其抗炎功效和潜在的临床应用。方法：采用逐层沉积法在可吸收缝线上涂覆AgNPs。采用扫描电镜（SEM）观察了缝线表面AgNPs的形态和分布。将1厘米的未包被缝线、包被抗生素缝线或包被agnps缝线分别置于LB琼脂板的大肠杆菌覆盖层上，以检测细菌抑制作用。然后将相应的缝合线用于小鼠回肠吻合。取吻合口观察组织炎症程度、细胞增殖及胶原沉积情况。此外，还采用破裂压力测试方法测试了材料的力学性能。结果：扫描电镜观察表明，AgNPs可以固定在缝线表面，且分布均匀。与其他组相比，agnps包被缝线的体外抗菌效果最好。随后的肠吻合模型免疫组化显示，agnps包被缝合组吻合口组织中炎症细胞（巨噬细胞和中性粒细胞）浸润明显减少，胶原沉积明显改善。愈合吻合口破裂压力测量进一步证实了包覆agnps缝线具有较好的力学性能。结论：我们的研究表明，agnps包被缝线可以改善吻合口愈合，因为它具有更好的力学性能，减少了炎症。(C) 2014爱思唯尔公司版权所有。
Background: Our previous studies have revealed that silver nanoparticles (AgNPs) had anti-inflammatory properties. In this study, we coated AgNPs onto the surface of absorbable suture, to further explore their anti-inflammatory efficacy and potential clinical application using an intestinal anastomosis model.Methods: Layer-by-layer deposition was used to coat AgNPs on absorbable sutures. Scanning electron microscopy (SEM) was conducted to observe the morphology and distribution of AgNPs on suture surface. 1 cm of either non-coated suture, suture coated with antibiotics or AgNPs-coated suture was placed on E. coli overlay of LB agar plates to test for bacterial inhibition. The respective sutures were then used for ileal anastomosis in mice. The anastomotic sites were harvested to investigate the degree of tissue inflammation and cell proliferation, as well as collagen deposition. Furthermore, burst pressure measurement was employed to test for mechanical properties.Results: SEM observation indicated AgNPs could be immobilized and distributed on suture surface evenly. AgNPs-coated suture had the best in vitro anti-bacterial efficacy when compared with other groups. Subsequent immunohistochemistry in the intestinal anastomosis model showed significantly less inflammatory cell infiltration (macrophage and neutrophil) and better collagen deposition in the anastomotic tissue in the AgNPs-coated suture group. Burst pressure measurement in healed anastomosis further confirmed that AgNPs-coated suture had better mechanical properties.Conclusion: Our study suggests that AgNPs-coated sutures can improve anastomosis healing due to better mechanical properties from reduced inflammation. (C) 2014 Elsevier Inc. All rights reserved.