The mannose-sensitive hemagglutinin of Vibrio cholerae promotes adherence to zooplankton
The mannose-sensitive hemagglutinin of Vibrio cholerae promotes adherence to zooplankton
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DOI：
10.1128/aem.67.7.3220-3225.2001
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发表时间：
2001-07-01
影响因子：
4.4
通讯作者：
Taylor, RK
中科院分区：
文献类型：
--
作者：
Chiavelli, DA;Marsh, JW;Taylor, RK
霍乱弧菌是霍乱的病原体，通常附着在浮游生物上，这一特性被认为有助于其在水生生境中的环境持久性。C：霍乱弧菌O 1 EITor生物型和霍乱弧菌O 139菌株产生称为甘露糖敏感血凝素（MSHA）的表面菌毛，而霍乱弧菌O 1经典生物型菌株不产生。虽然II型霍乱弧菌O 1经典型没有详细说明R ISI-LJ，但该基因存在并以与其他菌株相当的水平表达。自霍乱弧菌O 1 El Tor和霍乱弧菌O 139取代I.在过去的15年中，我们研究了MSHA在介导对浮游生物的粘附中的潜在作用。我们发现，V cholesterol O 1 EI Tor中的mshA突变显著减少，但没有消除，对浮游甲壳动物蚤状水蚤（Daphnia pulex）外骨骼的粘附。突变的影响是更明显的I：cholesterol O 139，基本上消除坚持。霍乱弧菌O 1经典mskA突变体的粘附性不受影响。结果表明，MSW是影响T cholesterol粘附浮游动物能力的一个因素，研究结果还表明，两种生物型的lr cholesterol O_1利用MSHA以外的因素来粘附浮游动物。MSHA和这些尚未定义的额外粘附因子的表达因血清群和生物型而异。
The bacterium Vibrio cholerae, the etiological agent of cholera, is often found attached to plankton, a property that is thought to contribute to its environmental persistence in aquatic habitats. The C: cholerae O1 EI Tor biotype and V cholerae O139 strains produce a surface pilus termed the mannose-sensitive hemagglutinin (MSHA), whereas V. cholerae O1 classical biotype strains do not. Although I I cholerae O1 classical does not elaborate R ISI-LJ, the gene is present and expressed at a level comparable to that of the other strains. Since V. cholerae O1 El Tor and V. cholerae O139 have displaced I. cholerae O1 classical as the major epidemic strains over the last fifteen Sears, we investigated the potential role of MSHA in mediating adherence to plankton, We found that mutation of mshA in V cholerae O1 EI Tor significantly diminished, but did not eliminate, adherence to exoskeletons of the planktonic crustacean Daphnia pulex. The effect of the mutation was more pronounced for I: cholerae O139, essentially eliminating adherence. Adherence of the V. cholerae O1 classical mskA mutant was unaffected. The results suggest that MSW is a factor contributing to the ability of T cholerae to adhere to plankton, The results also showed that both biotypes of lr cholerae O1 utilize factors in addition to MSHA for zooplankton adherence. The expression of MSHA and these additional, yet to be defined, adherence factors differ in a serogroup- and biotype-specific manner.