Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain.
Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain.
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DOI：
10.3201/eid2312.151146
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发表时间：
2017-12
影响因子：
11.8
通讯作者：
Fernández-Garayzábal JF
中科院分区：
文献类型：
--
作者：
Vela AI;Villalón P;Sáez-Nieto JA;Chacón G;Domínguez L;Fernández-Garayzábal JF
化脓性链球菌似乎几乎仅限于人类，很少有从动物身上分离出来的报告。我们提供了来自不同临床背景动物的15株化脓性链球菌的详细特征(EMM分型、脉冲场凝胶电泳[PFGE]和多位点序列分型[MLST])。我们还对红霉素的抗性机制、表型和毒力基因进行了研究。我们观察到两种emm类型：emm12(11株)和emm77(4株)。类似地，我们观察到2个遗传连锁，序列类型(ST)26和ST63。大多数分离株对大环内酯类药物耐药表型为MefA/ermB。根据emm-MLST-PFGE-毒力基因图谱组合，将分离株分为2个克隆：克隆1，表现为emm12-ST36-Pulsotype A-SpeG组合；克隆2，表现为emm77-ST63-Pulsotype B-Spec。我们的结果并不是决定性的表明动物可能是化脓性链球菌的新储存库，但表明了人类来源的化脓性链球菌分离株定植和感染动物的能力。
Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds. We also investigated erythromycin resistance mechanisms and phenotypes and virulence genes. We observed 2 emm types: emm12 (11 isolates) and emm77 (4 isolates). Similarly, we observed 2 genetic linages, sequence type (ST) 26 and ST63. Most isolates exhibited the M macrolide resistance phenotype and the mefA/ermB genotype. Isolates were grouped into 2 clones on the basis of emm-MLST-PFGE-virulence gene profile combinations: clone 1, characterized by the combined genotype emm12-ST36-pulsotype A-speG; and clone 2, characterized by the genotype emm77-ST63-pulsotype B-speC. Our results do not show conclusively that animals may represent a new reservoir of S. pyogenes but indicate the ability of human-derived S. pyogenes isolates to colonize and infect animals.
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影响因子：
5.2
作者：
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通讯作者：
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