Generation of breast cancer stem cells by steroid hormones in irradiated human mammary cell lines.
Generation of breast cancer stem cells by steroid hormones in irradiated human mammary cell lines.
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DOI：
10.1371/journal.pone.0077124
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nenoi M
中科院分区：
文献类型：
--
作者：
Vares G;Cui X;Wang B;Nakajima T;Nenoi M
暴露于电离辐射被证明会导致乳腺癌的风险增加。有强有力的证据表明，类固醇激素影响放射敏感性和乳腺癌的风险。肿瘤可以由癌症干细胞（CSC）的小亚群引发。为了评估类固醇激素对辐射诱导的乳腺癌风险的调节是否涉及CSC，我们通过流式细胞术测量了孕激素和雌激素治疗后辐照乳腺癌细胞系中CSC的比例。孕激素刺激扩增的CSC室在孕激素受体（PR）阳性的乳腺癌细胞和PR阴性的正常细胞。在MCF 10A正常上皮PR阴性细胞中，甾体酮治疗和辐射触发了癌症和干细胞相关microRNA调节（如miR-22和miR-29 c表达下调），这导致耐辐射肿瘤起始CSC的比例增加。
Exposure to ionizing radiation was shown to result in an increased risk of breast cancer. There is strong evidence that steroid hormones influence radiosensitivity and breast cancer risk. Tumors may be initiated by a small subpopulation of cancer stem cells (CSCs). In order to assess whether the modulation of radiation-induced breast cancer risk by steroid hormones could involve CSCs, we measured by flow cytometry the proportion of CSCs in irradiated breast cancer cell lines after progesterone and estrogen treatment. Progesterone stimulated the expansion of the CSC compartment both in progesterone receptor (PR)-positive breast cancer cells and in PR-negative normal cells. In MCF10A normal epithelial PR-negative cells, progesterone-treatment and irradiation triggered cancer and stemness-associated microRNA regulations (such as the downregulation of miR-22 and miR-29c expression), which resulted in increased proportions of radiation-resistant tumor-initiating CSCs.
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影响因子：
11.2
作者：
Charafe-Jauffret E;Ginestier C;Iovino F;Wicinski J;Cervera N;Finetti P;Hur MH;Diebel ME;Monville F;Dutcher J;Brown M;Viens P;Xerri L;Bertucci F;Stassi G;Dontu G;Birnbaum D;Wicha MS
通讯作者：
Wicha MS
影响因子：
4.8
作者：
Debnath, J;Muthuswamy, SK;Brugge, JS
通讯作者：
Brugge, JS
影响因子：
51.1
作者：
Badve, Sunil;Nakshatri, Harikrishna
通讯作者：
Nakshatri, Harikrishna
影响因子：
2.7
作者：
Gompel, A;Somai, S;Rostène, W
通讯作者：
Rostène, W
影响因子：
11.2
作者：
Cui, Xing;Oonishi, Kazuhiko;Okayasu, Ryuichi
通讯作者：
Okayasu, Ryuichi