CALCIMYCIN-INDUCED LENS EPITHELIAL-CELL APOPTOSIS CONTRIBUTES TO CATARACT FORMATION
CALCIMYCIN-INDUCED LENS EPITHELIAL-CELL APOPTOSIS CONTRIBUTES TO CATARACT FORMATION
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DOI：
10.1016/s0014-4835(95)80062-x
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发表时间：
1995-07-01
影响因子：
3.4
通讯作者：
SPECTOR, A
中科院分区：
文献类型：
--
作者：
LI, WC;KUSZAK, JR;SPECTOR, A
先前的研究表明，钙霉素在器官培养中会诱发白内障。为了研究这种诱导的机制，检查了钙霉素（钙离子载体，A23187）处理的大鼠晶状体中晶状体上皮细胞的活力。在用 5 μM 钙霉素孵育镜片期间，通过末端脱氧核苷酸转移酶 (TdT) 标记测定，处理 2 小时后发现凋亡上皮细胞。随着孵育时间的增加，凋亡细胞的百分比迅速上升。孵育12小时后，超过60%的晶状体上皮细胞发生凋亡。在这种治疗过程中还观察到了 c-fos 表达的延长，之前被证明是程序性细胞死亡的指标。 DNA片段化分析进一步证实TdT标记的细胞确实发生了凋亡。在相同的培养条件下，培养的晶状体在约30小时内逐渐失去透明度并变得完全不透明。由于脊椎动物晶状体仅包含单层上皮细胞，因此卡西霉素激活的这些细胞的凋亡很快会破坏晶状体上皮，损害下面纤维细胞的稳态并引发晶状体混浊的发展。 (C) 1995 学术出版社有限公司
Previous studies have shown that calcimycin induces cataract in organ culture. To investigate the mechanism of this induction, the viability of lens epithelial cells in calcimycin (calcium ionophore, A23187)-treated rat lenses were examined. During incubation of lenses with 5 mu M calcimycin, apoptotic epithelial cells were found after a 2-hr treatment as determined by terminal deoxynucleotidyl transferase (TdT) labeling. The percentage of apoptotic cells quickly rose as the incubation time increased. After a 12-hr incubation, more than 60% of the lens epithelial cells underwent apoptosis. Prolonged c-fos expression, previously shown to be an indicator of programmed cell death, was also observed during this treatment. DNA fragmentation assays further confirmed that the TdT labeled cells were indeed apoptotic. Under the same incubation conditions, the cultured lenses gradually lost transparency and became completely opaque in about 30 hr. Since the vertebrate lens contains only a single layer of epithelial cells, apoptotic death of these cells activated by calcimycin quickly destroys the lens epithelium, impairs homeostasis of the underlying fiber cells and initiates development of lens opacification. (C) 1995 Academic Press Limited