Function and subnuclear distribution of Rpp21, a protein subunit of the human ribonucleoprotein ribonuclease P
Function and subnuclear distribution of Rpp21, a protein subunit of the human ribonucleoprotein ribonuclease P
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DOI：
10.1017/s1355838201010469
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发表时间：
2001-08-01
期刊：
影响因子：
4.5
通讯作者：
Altman, S
中科院分区：
文献类型：
--
作者：
Jarrous, N;Reiner, R;Altman, S
Rpp 21是人核糖核酸酶P（RNase P）的一个蛋白亚基，它与酿酒酵母（Saccharomycescerevisiae）核糖核酸酶P（RNase P）的一个必需亚基Rpr2p具有同源性。Rpp 21是由位于主要组织相容性复合体（major histocompatibility complex）的I类基因簇中的一个基因编码的，与高度纯化的RNase P相关，并结合前体tRNA。Rpp21主要定位于核质中，但当以高水平表达时也在核仁和卡哈尔体中观察到。Rpp21前体mRNA中的内含子保留和剪接位点选择调节蛋白产物的核内分布及其与RNase P全酶的关联。我们的研究表明，包括核仁、核仁周围区室和Cajal小体在内的动态核结构都参与了人RNase P的产生和组装。
Rpp21, a protein subunit of human nuclear ribonuclease P (RNase P) was cloned by virtue of its homology with Rpr2p, an essential subunit of Saccharomyces cerevisiae nuclear RNase P. Rpp21 is encoded by a gene that resides in the class I gene cluster of the major histocompatibility complex, is associated with highly purified RNase P, and binds precursor tRNA. Rpp21 is predominantly localized in the nucleoplasm but is also observed in nucleoli and Cajal bodies when expressed at high levels. Intron retention and splice-site selection in Rpp21 precursor mRNA regulate the intranuclear distribution of the protein products and their association with the RNase P holoenzyme. Our study reveals that dynamic nuclear structures that include nucleoli, the perinucleolar compartment and Cajal bodies are all involved in the production and assembly of human RNase P.