Effects of dietary supplementation of an enzyme blend on the ileal and fecal digestibility of nutrients in growing pigs
Effects of dietary supplementation of an enzyme blend on the ileal and fecal digestibility of nutrients in growing pigs
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DOI：
10.2527/jas.2007-0262
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发表时间：
2008-07-01
影响因子：
3.3
通讯作者：
Pettigrew, J. E.
中科院分区：
文献类型：
--
作者：
Ji, F.;Casper, D. P.;Pettigrew, J. E.
本实验的目的是确定 β-葡聚糖酶-蛋白酶混合酶产品 (EBP) 对生长猪粪便消化率 (FD)、表观回肠消化率 (AID)、标准化回肠消化率和后肠消化率的影响。将 12 头回肠插管、正在生长的小猪 (38.2 +/- 0.5 kg) 安置在单独的代谢板条箱中，通过先前的饲料摄入分为 3 组，每组 4 头猪，并随机分配到 4 x 4 拉丁方设计的 3 个重复的方格（组）内的 4 个处理中的 1 个。处理为基础饮食（Basal）、基础饮食+ 0.05% EBP（0.05% EBP）、基础饮食+ 0.10% EBP（0.10% EBP），以及用于测量内源氨基酸的水解酪蛋白。基础日粮由玉米和豆粕组成，经计算含有 3.36 麦卡 ME/kg 和 1.1% 的总赖氨酸（按饲喂基础）。拉丁方各重复第一期的采食量为预备期4头猪最低采食量的85%，并在各方格内均衡。随后的每个时期，饲喂量增加 100 克/天。每个实验周期为14 d，其中饮食适应期4 d，粪便收集5 d，过渡期3 d，回肠收集2 d。每天以相同的 12 小时间隔连续收集回肠流出物。与饲喂基础饲料的猪相比，饲喂 EBP 的猪的 DM、OM、能量、CP、非纤维碳水化合物、总膳食纤维、不溶性膳食纤维、酸水解脂肪、灰分、Ca 和 P 的 FD 增加 (P < 0.05)。通过在日粮中添加 0.05% 或 0.10% 的 EBP，NDF 和半纤维素的 AID 增加（P < 0.05），但 DM 和能量的 AID 没有增加。饲喂 EBP 的猪的酸水解脂肪的 AID 往往高于饲喂基础饲料的猪 (P = 0.051)。大多数氨基酸的回肠消化率不受治疗影响，但 EBP 降低了蛋氨酸、丙氨酸和丝氨酸的表观消化率和标准化消化率（P < 0.05）。饲喂 EBP 的猪的半纤维素 FD 和 AID 之间的差异低于饲喂 Basal 的猪（P < 0.05）。这些结果表明，饲喂生长猪的EBP改善了生长猪的DM、OM、能量、CP、非纤维碳水化合物、总膳食纤维、酸水解脂肪、Ca和P的FD以及NDF和半纤维素的AID，但在以玉米-豆粕为基础的日粮中添加EBP并没有改善氨基酸的标准化回肠消化率。
The objective of this experiment was to determine the effect of a beta-glucanase-protease enzyme blend product (EBP) on fecal digestibility (FD), apparent ileal digestibility (AID), standardized ileal digestibility, and digestibility in the hindgut of growing pigs. Twelve ileal-cannulated, growing barrows (38.2 +/- 0.5 kg) were housed in individual metabolism crates, blocked by previous feed intake into 3 groups with 4 pigs each, and randomly assigned to 1 of 4 treatments within a square (group) of 3 replications of 4 x 4 Latin square design. Treatments were basal diet (Basal), Basal + 0.05% of EBP (0.05% EBP), Basal + 0.10% of EBP (0.10% EBP), and hydrolyzed casein for measurement of endogenous amino acids. The Basal diet consisted of corn and soybean meal and was calculated to have 3.36 Mcal of ME/kg and 1.1% of total lysine, as-fed basis. Feed intake of each replicate of the Latin square during the first period was 85% of the minimum feed intake of the 4 pigs during the preliminary period and was equalized within each square. The feeding level was increased by 100 g/d in each subsequent period. Each of the experimental periods was 14 d, including 4 d of dietary adaptation, 5 d of fecal collection, 3 d of transition period, and 2 d of ileal collection. Ileal effluents were collected continuously for the same 12-h interval each day. Pigs fed the EBP demonstrated increased ( P < 0.05) FD of DM, OM, energy, CP, nonfiber carbohydrate, total dietary fiber, insoluble dietary fiber, acid-hydrolyzed fat, ash, Ca, and P compared with pigs fed Basal. The AID of NDF and hemicellulose was increased (P < 0.05) by supplying the EBP either at 0.05 or 0.10% in the diets, but AID of DM and energy was not increased. The AID of acid-hydrolyzed fat tended to be greater (P = 0.051) for the pigs fed the EBP than for those fed Basal. Ileal digestibility of most amino acids was not affected by treatment, but the EBP reduced the apparent and standardized digestibility of methionine, alanine, and serine (P < 0.05). The difference between FD and AID of hemicellulose was lower (P < 0.05) for the pigs fed the EBP than for those fed Basal. These results demonstrated that the EBP fed to growing pigs improved the FD of DM, OM, energy, CP, nonfiber carbohydrate, total dietary fiber, acid-hydrolyzed fat, Ca, and P, and the AID of NDF and hemicellulose, but the standardized ileal digestibility of amino acids was not improved by supplying the EBP in corn-soybean meal-based diets of growing pigs.