Secretory pancreatic stone protein messenger RNA. Nucleotide sequence and expression in chronic calcifying pancreatitis.
Secretory pancreatic stone protein messenger RNA. Nucleotide sequence and expression in chronic calcifying pancreatitis.
复制标题
分泌性胰石蛋白信使RNA。
DOI：
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发表时间：
1989
影响因子：
15.9
通讯作者：
J. Dagorn
中科院分区：
文献类型：
--
作者：
D. Giorgi;J. Bernard;S. Rouquier;J. Iovanna;H. Sarles;J. Dagorn
胰腺结石蛋白及其分泌型(PSP-S)是碳酸钙晶体生长的抑制因子，可能参与稳定胰液。我们建立了PSP-S基因的结构，并对其在慢性钙化性胰腺炎中的表达进行了检测。从人胰腺c DNA文库中克隆了编码PSP-S前蛋白的c DNA。核苷酸序列分析表明，该基因除pSP-S基因5‘端外，其余序列均由pSP-S基因第一外显子测序得到。该基因全长775个核苷酸，包括80个核苷酸的5‘非编码区和197个核苷酸的3’非编码区，分别连接在一个约125个核苷酸的聚(A)尾部。它编码166个氨基酸的前蛋白，其中包括22个氨基酸的前肽。未发现PSP-S与其他胰腺蛋白的全序列同源性。然而，在COOH末端区域观察到了与几种丝氨酸蛋白酶的一些同源性。比较慢性胰腺炎和对照组胰腺组织中PSP-S、胰蛋白酶原、胰凝乳酶原和脂酶的表达水平。CCP组PSP-S基因表达水平较对照组降低3倍，其余指标无明显变化。结论：慢性阻塞性肺疾病患者PSP-S基因表达特异性降低。
The pancreatic stone protein and its secretory form (PSP-S) are inhibitors of CaCO3 crystal growth, possibly involved in the stabilization of pancreatic juice. We have established the structure of PSP-S mRNA and monitored its expression in chronic calcifying pancreatitis (CCP). A cDNA encoding pre-PSP-S has been cloned from a human pancreatic cDNA library. Its nucleotide sequence revealed that it comprised all but the 5' end of PSP-S mRNA, which was obtained by sequencing the first exon of the PSP-S gene. The complete mRNA sequence is 775 nucleotides long, including 5'- and 3'- noncoding regions of 80 and 197 nucleotides, respectively, attached to a poly(A) tail of approximately 125 nucleotides. It encodes a preprotein of 166 amino acids, including a prepeptide of 22 amino acids. No overall sequence homology was found between PSP-S and other pancreatic proteins. Some homology with several serine proteases was observed in the COOH-terminal region, however. The mRNA levels of PSP-S, trypsinogen, chymotrypsinogen, and colipase in CCP and control pancreas were compared. PSP-S mRNA was three times lower in CCP than in control, whereas the others were not altered. It was concluded that PSP-S gene expression is specifically reduced in CCP patients.
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
White,BA;Bancroft,FC
通讯作者：
Bancroft,FC
使用人类补体第四种成分 (C4) 的 cDNA 克隆来分析豚鼠 C4 的遗传缺陷。
DOI：
10.1073/pnas.80.17.5387
发表时间：
1983
影响因子：
11.1
作者：
Whitehead,AS;Goldberger,G;Woods,DE;Markham,AF;Colten,HR
通讯作者：
Colten,HR
胰腺和胆道 CaCO3 结石的发病机制：用 Ca 电极测定方解石的溶解度积 (Ksp)。
DOI：
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发表时间：
1985
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Moore,EW;Verine,HJ
通讯作者：
Verine,HJ